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Lecture 5

Fibroblasts....
Secrete collagen
Fuse to for muscle fibers
Extend axons
Form extensive sheets
Embryonic skeletal muscles....
Secrete collagen
Fuse to for muscle fibers
Extend axons
Form extensive sheets
Nerve Cells
Secrete collagen
Fuse to for muscle fibers
Extend axons
Form extensive sheets
Epithelial Cells
Secrete collagen
Fuse to for muscle fibers
Extend axons
Form extensive sheets
What membranes break up in the fractionation of cells?
Plasma Membrane
ER Membranes
Both
What is distinctive in the thick extract from the suspension of cells?
Size
Charge
Density
All of the above
Nuclei precipitates at....
1000g
10000g
100000g
100g
Mitochondria precipitates at
1000g
10000g
100000g
100g
Lysosomes precipitate at
1000g
10000g
100000g
100g
Microsomes from the ER precipitate at
1000g
10000g
100000g
100g
Ribosomes precipiates at
1000g
10000g
100000g
100g
Proteins produced by the immune system as a defense against infection.
Anitbodies
Antigens
Tissues
Electric Field
The antibodies all have the same binding sites.
True
False
Different antibodies recognize various parts of the antigen molecule.
ImmuneFlorescence
Immunehistochemistry
Antigen/Antibody
Antigenic Determinant
Recognize & Binds specifically to certain component within the cell.
Primary Antibody
Secondary Antibody
Recognizes and binds specifically to primary antibody.
Primary Antibody
Secondary Antibody
TAGGED with fluorescent dyes or produce reaction outcome color.
Primary Antibody
Secondary Antibody
What is the correct sequence of steps in the study of protein expressions?
Collect Samples -> Homogenize Tissue -> Inject Protein -> Use Electric Field -> Add Molecular Ladder -> Transfer Protein Bands -> Add Primary/Secondary Antibody -> Compare
Collect Samples -> Homogenize Tissue -> Inject Protein -> Use Electric Field -> Add Molecular Ladder -> Add Primary/Secondary Antibody-> Transfer Protein Bands -> Compare
What is the protein for protein expressions injected in?
Polyacramide Gel
Charged Amino Acids
Nitrocellulose Membrane
Antibody-rich serum
Proteins migrate according to....
Charge
Size
Molecular Weight
All of the Above
What does the molecular ladder give?
Negative sample
Positive sample
Where are the protein bands transferred to?
Polyacramide Gel
Charged Amino Acids
Nitrocellulose Membrane
Antibody-rich serum
The tag added to the secondary antibodies is to visualize the.....
Primary Antibody
Nucleus
Protein Bands
Protein migrates at a rate that depends on its...
Net charge
Size
Shape
All of the above
What is used as the matrix that proteins migrate through?
Polyacramide Gel
Charged Amino Acids
Nitrocellulose Membrane
Antibody-rich serum
Proteins possess a net positive or negative charge depending on the mixture of...
Polyacramide Gel
Charged Amino Acids
Nitrocellulose Membrane
Antibody-rich serum
What is used to heat proteins?
A) SDS
B) Mercaptoethanol
C) Polycrylamide-Gel
D) A + B
Regarding Antibody-Antigen reaction, mark the wrong statement.
Primary antibody is raised in-order to recognize specific component of the cell.
Secondary antibody is raised to recognize primary antibody.
A tag may be attached to primary antibody to locate it within the tissue.
Antibody-Antigen reaction is used to study regulation of proteins.
Antibody-Antigen reaction is used in immuniflourescent studies.
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