Lecture 5

Fibroblasts....

Secrete collagen

Fuse to for muscle fibers

Extend axons

Form extensive sheets

Embryonic skeletal muscles....

Secrete collagen

Fuse to for muscle fibers

Extend axons

Form extensive sheets

Nerve Cells

Secrete collagen

Fuse to for muscle fibers

Extend axons

Form extensive sheets

Epithelial Cells

Secrete collagen

Fuse to for muscle fibers

Extend axons

Form extensive sheets

What membranes break up in the fractionation of cells?

Plasma Membrane

ER Membranes

Both

What is distinctive in the thick extract from the suspension of cells?

Size

Charge

Density

All of the above

Proteins produced by the immune system as a defense against infection.

Anitbodies

Antigens

Tissues

Electric Field

Different antibodies recognize various parts of the antigen molecule.

ImmuneFlorescence

Immunehistochemistry

Antigen/Antibody

Antigenic Determinant

Recognize & Binds specifically to certain component within the cell.

Primary Antibody

Secondary Antibody

Recognizes and binds specifically to primary antibody.

Primary Antibody

Secondary Antibody

TAGGED with fluorescent dyes or produce reaction outcome color.

Primary Antibody

Secondary Antibody

What is the correct sequence of steps in the study of protein expressions?

Collect Samples -> Homogenize Tissue -> Inject Protein -> Use Electric Field -> Add Molecular Ladder -> Transfer Protein Bands -> Add Primary/Secondary Antibody -> Compare

Collect Samples -> Homogenize Tissue -> Inject Protein -> Use Electric Field -> Add Molecular Ladder -> Add Primary/Secondary Antibody-> Transfer Protein Bands -> Compare

What is the protein for protein expressions injected in?

Polyacramide Gel

Charged Amino Acids

Nitrocellulose Membrane

Antibody-rich serum

Where are the protein bands transferred to?

Polyacramide Gel

Charged Amino Acids

Nitrocellulose Membrane

Antibody-rich serum

The tag added to the secondary antibodies is to visualize the.....

Primary Antibody

Nucleus

Protein Bands

Protein migrates at a rate that depends on its...

Net charge

Size

Shape

All of the above

What is used as the matrix that proteins migrate through?

Polyacramide Gel

Charged Amino Acids

Nitrocellulose Membrane

Antibody-rich serum

Proteins possess a net positive or negative charge depending on the mixture of...

Polyacramide Gel

Charged Amino Acids

Nitrocellulose Membrane

Antibody-rich serum

What is used to heat proteins?

A) SDS

B) Mercaptoethanol

C) Polycrylamide-Gel

D) A + B

Regarding Antibody-Antigen reaction, mark the wrong statement.

Primary antibody is raised in-order to recognize specific component of the cell.

Secondary antibody is raised to recognize primary antibody.

A tag may be attached to primary antibody to locate it within the tissue.

Antibody-Antigen reaction is used to study regulation of proteins.

Antibody-Antigen reaction is used in immuniflourescent studies.

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Lecture 5

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