DNA and Genetic Engineering Quiz
DNA and Genetic Engineering Quiz
Test your knowledge on DNA, genetic engineering, and the various processes involved in creating DNA profiles. This quiz covers key concepts such as gel electrophoresis, restriction enzymes, and plasmids.
Challenge yourself with questions about:
- DNA profiling
- Gel electrophoresis techniques
- Applications of recombinant DNA technology
Which of the following could be collected at a crime scene to create a DNA profile for a suspect?
A blood sample.
Imprints of shoes in mud outside of the broken door.
A letter of admission written and signed by the suspect saying “it was me!” on the computer at the crime scene.
Fingerprints.
Once a sample of DNA has been fragmented, which process is used to separate the fragments?
Recombinantion
Plasmids
Restriction
Electrophoresis.
DNA fragments move through the gel a distance that is inversely proportional to their length. Which of the following fragments would move furthest?
1100 base pairs
1500 base pairs
900 base pairs
4000 base pairs
A sample of DNA is fragmented by restriction enzymes, Fragment 1 = 1000 bp, Fragment 2 =2000 bp, Fragment 3 = 3000 bp, Fragment 4 4000 bp. Following electrophoresis how would the fragment appear in the gel?
� electrode 4, 1, 3, 2, + electrode
+ electrode 3, 2, 1, 4, - electrode
� electrode 4, 3, 2, 1, + electrode
+ electrode 4, 3, 2, 1, - electrode
Which of the following represents the correct sequence of steps in the process of gel electrophoresis?
Load the gel with samples, insert the comb, electrophorese the gel, pour the gel
Pour the gel, insert the comb, load the gel with samples, electrophorese the gel
Insert the comb, pour the gel, load the gel with samples, electrophorese the gel
Insert the comb, load the gel with samples, pour the gel, electrophorese the gel
What is the function of a restriction enzyme in recombinant technology?
Cut DNA into fragments at specific locations
Separate fragments of DNA by their length and electrical charges
Make millions of copies of a specific segment of DNA
Link together newly joined fragments of DNA
Carry DNA into a new cell
What is the function of plasmids in genetic engineering?
Carry recombinant DNA into a new cell
Make millions of copies of a specific segment of DNA
Separate fragments of DNA by their length and electrical charges
Cut DNA into many fragments
Link together newly joined fragments of DNA
What is the function of gel electrophoresis in genetic engineering?
To separate fragments of DNA by their length and electrical charges
To carry recombinant DNA into a new cell
To make millions of copies of a specific segment of DNA
To cut DNA into many fragments
To link together newly joined fragments of DNA
A molecule of DNA that contains genes from more than one source is described as
DNA polymerase
Recombinant DNA
Complimentary DNA
Messenger RNA
The bands seen is a gel following electrophoresis of a DNA sample to create a DNA profile represent:-
DNA fragments of equal length
Recombinant DNA from all of the individuals
Proteins of equal size
Cells from all of the individuals tested
What is a plasmid?
The human gene for making plasma.
An enzyme that makes DNA
An enzyme that cuts DNA
A small ring of DNA separate from the bacterial chromosome
In which organelle would DNA be located in a strawberry cell?
The nucleus
In the seeds
DNA precipitates and becomes visible after adding cold ethanol.
In the fruit
Which of the following plates would you expect to see glowing colonies of transformed bacteria?
- DNA LB/amp
+DNA LB/amp
All of the above
+DNA LB/amp/ara
Assuming that the plasmid was successfully taken up by the bacteria, what two external factors are necessary for the bacteria to fluoresce?
UV light and ampicillin
Ampicillin and warm water
UV light and arabinose
Ampicillin and arabinose
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