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Anti-doping

Blood-doping can involve autologous blood, which is

Blood of the same blood type

Blood of a different blood type

Someone else's blood

Your own blood

How many whereabouts failures constitutes a violation

An ELISA is a type of which analytical technique

Western blotting

Chromotography

Plate-based immunoassay

A detection antibody is added and binds to the analyte-capture antibody complex, what is then added to bind to the detection antibody

Substrate

Mobile phase

Conjugated antibody

Enzymes

What is done between each stage of an ELISA

Excess liquid is removed and plate wells are washed

An enzyme is added to remove waste

Alcohol is used to denature proteins

The pH of the plate is measured

What must be done to proteins in a sample to prepare them for an immunoblot

Hydrolysed

Denatured

Conjugated

Glycosylated

In which direction is the charge orientated to transfer the sample from the electrophoresis gel to the blotting membrane

Gel: +ve, membr: -ve

Gel: -ve, membr: +ve

Gel: neutral, membr: neutral

Gel: +ve, membr: +ve

What does the primary antibody bind to on the protein in western blotting

Conjugated antibody

Substrate

Prosthetic group

Epitope

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