GENETICS or should i say death

Which steps below are unique to eukaryotic gene expression, but not to prokaryotic gene expression?

Transcription

RNA splicing

Addition of 5'CAP and 3'PolyA tails

RNA splicing and Addition of 5'CAP and 3'PolyA tails

If we examine the structure of a eukaryotic gene, which of the following will be found in its DNA sequence?

Which of the following statement is TRUE regarding RNAs in eukaryotic gene expression?

They are all transcribed by a single RNA polymerase

They are all used for translation

There are 5 different types of RNA molecules

They are all transported out of the nuclei after processing

According to the experiment in Figure 11.19, the R loop is the evidence for an intron in the mouse beta-globin gene. In this experiment, the RNA (as in green) complements with ________ in the DNA strand?

The template strand of the exon

The non-template strand of the inton

The non-template strand of the exon

The template strand of the intron

According to the textbook, translation follows the same steps in prokaryotes and eukaryotes. The large ribosomal subunits contain 23S or 28S rRNA, which serves as the enzyme for peptide bond formation. This enzymatic activity is known to be _______________.

Peptidal tansferase

Aminoacyl-tRNA synthetase

Elongation factor

Protein polymerase

According to the central dogma of biology, DNA is ________ into mRNA, and then is __________ into polypeptide

translated...translated

transcribed..translated

translated...transcribed

If a mutation occurs to the initiation codon AUG, what will happen?

No transcription in prokaryotes only

No transcription in both prokaryotes and eukaryotes

No translation in both eukaryotes and prokaryotes

Nothing will happen

Which type of RNA molecules are part of the spliceosomes in eukaryotes?

tRNA

snRNA

miRNA

rRNA

ccording to Beadle and Tantum's experiment, which of the following is correct?

If one mutant strain can't make folic acid, one needs to supplement it in the minimal medium to grow bacteria

Wildtype strains only grow on complete medium, but not on minimal medium

Mutant strains grow on both complete and minimal medium

If one mutant strain can't make niacin, it will not grow on the complete medium

The genetic code is triplet. So how many reading frames are there for a given mRNA sequence?

genetics is hard

Splicing signals (dinucleotide sequence in the intron)

Promoter

Start codon also known as initiator codon

5'CAP sequence

ou see partial sequence of a gene as follows (non template strand shown) ...TATAAA(8nts)GGCCAAGGAGCCGCCATGGAACCTGAAG...AAAAAAAAAAAAA... +1 initiation site is underlined. Is this a prokaryotic or eukaryotic gene? Why?

Eukaryotic gene because it has a TATA box

Prokaryotic gene because it has the Shine Dalgarno sequence

Prokaryotic gene because it has -10 AT rich region

Eukaryotic gene because it has poly A tail