Recombinant DNA Technology

what is Gene Cloning
Making a copy of piece of DNA or gene of interest
Formation of new gene or DNA
Production of different genes in same host cell
Transformation of DNA in a foreign gene a
The first step in rDNA Technology is
Transformation
Insertion of foreign DNA in a plasmid vector
selection and Isolation of gene of interest
Selection of vector
A circular DNA present in bacteria, used as a vector is known as
Bacteriophage
Cosmid
Phagemid
Plasmid
The enzymes used to make cut in a DNA molecule or vector at specific site are known as
Restriction exonucleases
Restriction endonucleases
Nuclease
Polynucleotide kinase
The enzyme that joins two ends of DNA molecule is known as
Ligase
Kinase
Lyase
Restriction endonuclease
A vector with foreign gene insert is known as
Foreign DNA
Plasmid DNA
Recombinant DNA
Cp DNA
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