most translational regulatory mechanisms affect initiation of translation
cap-dependent initiation is a prevalent initiation mechanisms in eukaryotes
global translation is activated upon stress
eIF4G proteolysis upon viral infection and apoptosis inhibits cap-dependent and activates IRES-driven translation
Mark TRUE statements:
4E-BP proteins activate cap-dependent translation
miRNAs repress mRNA by recruiting deadenylation complexes
miRNAs cause translational repression, but not mRNA deadenylation.
Select TRUE statements about methods to monitor translation:
RNA-seq and MS (mass spectrometry)-based methods allow to quantify translation genome-wide
RNA-seq and MS (mass spectrometry)-based methods allow to quantify translation on single RNA molecules and in real time
Imaging-based methods allow to identify translation sites within the cell
These methods to measure translation are based on RNA-seq:
puro-PLA
BONCAT
Ribotag/TRAP
Ribosome profiling
These methods to measure translation are based on labeling of nascent peptides with highly reactive aminoacid analogs or an analog of aminoacylated tRNA:
TRICK
Puro-PLA
SunSET/ribopuromycylation
BONCAT/QuaNCAT
Pulsed SILAC (pSILAC)
FUNCAT/FUNCAT-PLA
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