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Translational control lectures

Mark TRUE statements:

Most translational regulatory mechanisms affect initiation of translation

Cap-dependent initiation is a prevalent initiation mechanisms in eukaryotes

Global translation is activated upon stress

EIF4G proteolysis upon viral infection and apoptosis inhibits cap-dependent and activates IRES-driven translation

Mark TRUE statements:

4E-BP proteins activate cap-dependent translation

MiRNAs repress mRNA by recruiting deadenylation complexes

MiRNAs cause translational repression, but not mRNA deadenylation.

Select TRUE statements about methods to monitor translation:

RNA-seq and MS (mass spectrometry)-based methods allow to quantify translation genome-wide

RNA-seq and MS (mass spectrometry)-based methods allow to quantify translation on single RNA molecules and in real time

Imaging-based methods allow to identify translation sites within the cell

These methods to measure translation are based on RNA-seq:

Puro-PLA

BONCAT

Ribotag/TRAP

Ribosome profiling

These methods to measure translation are based on labeling of nascent peptides with highly reactive aminoacid analogs or an analog of aminoacylated tRNA:

TRICK

Puro-PLA

SunSET/ribopuromycylation

BONCAT/QuaNCAT

Pulsed SILAC (pSILAC)

FUNCAT/FUNCAT-PLA

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