HPCT: Dehydration and Clearing

Dehydrating agent to tissue ratio

Fixative to tissue ratio

Most common dehydrating agent

Best dehydrating agent

Slow dehydrating agent.

Can cause blindness

Preserves negri bodies

Fast dehydrating agent

Wrap the tissue with gauze

Tissues can be left for long periods of time without affecting the staining properties of the specimen.

Used to dehydrate smears producing minimal shrinkage

Toxic when ingested or inhaled

Cellosolve is combustible at what temp?

An indicator of water bath content of the last bath.

What color indicates presence of water?

Indicator for dehydration

Chloroform may cause ______.

Recommended for very delicate tissues.

Clearing time for cedarwood oil

Clearing time for chloroform

Most rapid clearing agent

Clearing time for xylol

Clearing time for toluene

Clearing agent that causes minimum shrinkage

Clearing agent recommended for CNS tissue and cytology

Dehydrating agent recommended for CNS

Similar to chloroform but cheaper

Substitute for xylol or benzene

Recommended for urgent biopsies

Benze may cause ________.