Micro chapter 9-12

The use of living cells to make useful products
Biotechnology
Genetic engineering
DNA cloning
RRecombinant DNA Technology
Anyalyzed DNA sequences to reunite children with relatives
Mary-claire king
Mitichondrial DNA
DNA cloning
Clone gene for specific proteins
Vaccine Production
Cheese production
Transgenic
Chymosin ( enzyme from stomachs of calves
Chese production
Vaccine production
Transgenic
Engineered plants and animals
Tansgenic
Bacillus thuringiensis
Yeast
The process of determining the nucleotide sequence of a DNA molecule
DNA cloning
DNA sequencing
DNA probes
What locate specific nucleotide sequences
DNA cloning
DNA sequencing
DNA probes
What allows detectiion of clonies that have specific sequence of DNA
DNA cloning
DNA probes
Colony Blotting
Bacteria classified by shape
Ferdinand Cohn
Sigurd Orla-Jensen
Albert Kluyver
Relationships determined by comparing physical traits, nucleotide sequences
Roger Stanier
Ferdinand Cohn
Carl Woese
Prokaryotes divided into two major groups based upon ribosomal RNA sequences
Carl Woese
Roger stanier
Ferdinand Cohn
The science that studies organisms to arrange them into groups, or taxa
Taxonomy
Species
Phylogeny
What is evolutionary relatedness
Phylogeny
Taxonomy
Isolates
What can be changed by the environment
Serology
Plantae
Phenotype
What quickly determines size, shape, staining characteristics
Culture characteristics
Metabolic capabilities
Microscopic morphology
Streptococci colonie generally fairly small
Microscopic morphology
Culture characteristics
Serology
Biochemical tests provide more certainty of identification
Serology
Fatty acid analysis
Metabolic capabilities
Uses antibodies to detect antegens
Fatty acid analysis
Serology
Culture characteristics
Measurment of diffrent fatty acids
Culture characteristics
Fatty acid analysis
Metabolic capabilities
Often red at 22 degress Celcious
Serratia marcescens
Streptococcus pyogenes
Pseudomonas aeruginosa
Often produces green pigment with a fruity odor
Serratia marcescens
Streptococcus pyogenes
Pseudomonas aeruginosa
Strep throat and yeilds B-hemolytic colonies on blood agar
Serratia marcescens
Streptococcus pyogenes
Pseudomonas aeruginosa
Group with characteristic pattern : brovar or biotype
Antibiograms
Biochemical typing
Serological typing
Group with characteristic antigens : serovar or serotype
Antibiograms
Biochemical typing
Serological typing
Antibiotic susceptibility patterns
Biochemical typing
Antibiograms
Serological typing
Refers to feeding
Taxic
Troph
Hetero
Refers to movement in response to an environmental cue
Taxic
Aerobic
Troph
Inorganic
Chemo
Organo
Litho
Chemically-based
Litho
Hetero
Chemo
Organic, containing carbon
Organo
Aerobic
Auto
In the absence of oxygen
Anaerobic
Aerobic
Hetero
In the present of oxygen
Aerobic
Anerobic
Auto
Self
Hetero
Auto
Litho
Non-self
Auto
Hetero
Anaerobic
A group of Archaea that generate energy by breaking down h2 gas
Chemolithotrophs
Methonogens
Chemoorganotrophs
Break down organic chemicals for energy using fermentation
Chemooganotrophs
Chemolithotrophs
Methonogens
Break down reduced inorganic chemicals for energy
Chemolithotrophs
Chemoogantrophs
Anoxygenic phototrophs
Bacteria forming a resting stage are typically found in soil often as endospores
Sulfur
Habitat
Facultative
Heterotrophic organisms; chitin in cell wall
Fungi
Algae
Protozoa
Simple autotrophs
Fungi
Algae
Protists
Microscopic heterotrophs that are not fungi
Algae
Protozoa
Protists
Eukaryotes that are not fungi, plants, animals
Protists
Protozoa
Protozoa
Fungi: molds, yeast, mushrooms
Mycology
Saprophytic
Morphology
Nutrients from dead or decaying matter
Saprophytic
Morphology
Mycology
Single celled fungi
Mold
Yeasts
Mushrooms
Filamentous fungi
Yeast
Mold
Mushrooms
Reproductive structure of certain fungi
Morphology
Mycology
Saprophytic
Vidible mass of hyphae
Mycelium
Haustoria
Lichens
Protrude into host cells in parasitic fungi
Haustoria
Rhizoids
Mycelium
Can grow as single yeast cells or multicellular mycelia can also grow in human diseases
Dimorphic fungi
Lichens
Parasitic fungi
May anchor to substrate in saprophytic fungi
Haustoria
Rhizoids
Lichens
Alga and fungus
Lichens
Rhizoids
Mychorrhizas
Vital for plants and roots
Lichens
Mychorrhizas
Spore
Reproductive cells from sexually or asexually
Spore
Mychorrihizas
Mycoses
Often named after causative agent
Mycoses
Aspergillus
Fungal spore
Incorporate silicon dioxide into cell walls
Diatoms
Myocese
Algae
Major producer of oxygen
Algae
Algal blooms
Gonyaulax
Algal blooms of dionflagellates
Red tides
Gonyaulax
Parabasalids
Produces neurotoxins
Gonyaulax
Parabasalids
Red tides
Upwelling of nutrients and warmer temperatures, fertilizer runoff, untreated sewage
Red tides
Algal blooms
Algae
Live within host ( EX> termites)
Parabasalids
Malaria
Protozoa
African sleeping sickness
Leishmania major
Trypanosome brucei
Entamoeba histolytica
Entamoeba histolytica causes diarrhea in humans
True
False
Is swimmers itch caused by flukes
False
Ture
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