introduction to capillary electrophoresis
Capillary Electrophoresis Mastery Quiz
Test your knowledge on capillary electrophoresis with this comprehensive quiz designed for students, teachers, and professionals alike. From fundamental concepts to advanced applications, this quiz covers a broad range of topics that will enhance your understanding of this essential analytical technique.
Features of the quiz include:
- 80 diverse questions
- Multiple choice format
- Score tracking for performance assessment
Capillary electrophoresis is considered ………………….. technique.
Spectrophotometric
Spectrofluorimetric
Chromatographic
Separation
The movement of the analyte in CE is NOT occur ……….
Through a conductive medium,
In aqueous buffer
Under the effect of osmotic pressure
In the absence of any other effects, cations migrate toward the
Positively charged electrode
Anode
Cathode
In the absence of other effects, anions migrate toward
Negatively charged electrode
Anode
Cathode
The movement of the analyte in CE is NOT occur ……….
Through a conductive medium,
In aqueous buffer
Under the effect of osmotic pressure
The faster ions migrate have
High charge and small size,
A low charge-to-size ratio,
Large size and low charge
High charge and large size
Neutral species in capillary zone electrophoresis using uncoated silica capillary …….
Is affected by the electric field
remain stationary
Move toward cathode
move toward anode
CE have higher …………….. than HPLC methods.
analysis time
Solvent consumption
Sensitivity
Separation power
The cost in HPLC is …………….. CE methods.
higher than
Lower than
Similar to
CE ……………than HPLC
Is much more robust
Is more expensive
Has more parameters require optimization
Solvent consumption in CEis ………… HPLC multiple choice
very larger than
Very smaller than
Similar to
CE is currently ………….. HPLC. Multiple choice
Had the same robustness as
Much more robust than
Much less robust than
All of the followings are advantages of CE over HPLC except ………………… multiple choice
More efficient
Shorter analysis time
Cheaper columns
Higher sensitivity
Which of the followings is not an advantages of CE over HPLC ………………… multiple choice
More efficient
More robust
Shorter analysis time
All of the followings are disadvantages of CE compared to HPLC except ……………… multiple choice
Less robust
More expensive
Less sensitive
Which of the followings is a disadvantages of CE compared to HPLC ……………… multiple choice
Less sensitive
More expensive
Consume large volume of solvents
The capillary in CE should be filled with ……………. Multiple choice
An aqueous solution containing electrolyte
Organic solvent such as ethanol
Non aqueous solution containing buffer
Aqueous solution with high salt content
The two forces which are responsible for separation in CE are ……..
Capillary action and osmotic pressure
Osmotic pressure and electrophoretic mobility
Electroosmotic flow and electrophoretic mobility
Pressure and capillary action
The electrophoretic mobility (μ) of the substance increase by increasing ……..
The charge on the ion
The ion radius
Viscosity
CE is similar to other types of chromatography in that the……….. The greater its mobility
Greater the charge on the ion
Larger the ion
More closely an ion approximates to a sphere
The driving force in capillary electrophoresis is ………….
High pressure
Capillary action
High voltage
Not present
The analysis in capillary electrophoresis is carried out in ……………….
Capillary gas column
Stainless column
Narrow glass tube
Cuvette
The pH of the solution can NOT affect the ……….. Of the analyte.
Molecular shape
Mobility
Ionization
pKa
Separation of two drugs having similar shape and size but with different pKa values, can be obtained bycontrolling ………………
pH of the medium
The viscosity of the solution
The voltage
The buffer strength
For the analysis of basic drugs,the biggest numerical difference in mobility is obtained when multiple choice
PH = pKa of the weaker base
PH = pKa of the stronger base
PH =1/2 pKa of the weaker base
PH = 1/2 pKa of the stronger base
If two basic drugs possess different pKa, ……….. mobility(s) at full charge ionization. Multiple choice
The drug with lower pka will has higher
They will have the same
The drug with higher pka will has higher
The compound that has the lowest mobility at pH 9.55 in capillary electrophoresis is ….. Multiple choice
Doxepin (pKa=9.0)
Imipramine (pKa=9.5)
Desimipramine (pKa=10.2)
The faster compound at pH 9.55 in capillary electrophoresis is ……………….. Multiple choice
Doxepin (pKa=9.0)
Imipramine (pKa=9.5)
Desimipramine (pKa=10.2)
Which of the following drugs (codeine pka 8.2 and morphine pka 7.9) will have the highest mobility? Multiple choice
Codeine at pH = 6.0
Codeine at pH = 7.9
Morphine at pH = 8.2
At pH 4.0 - 9.0 the silanol groups on glass caplillary wallmultiple choice
Bear a negative charge
Have a positive charge
Is neural
The amount of negative charge on the silica capillary wall is dependent on………….. Multiple choice
The applied voltage
Concentration of the analyte
PH of the medium
The charge on the uncoated silica capillary is neutralized by ………. Multiple choice
Anions in the running buffer
Cations in the running buffer
Sodium inside silica
Analyte ions
When a potential is applied across the uncoated silica capillary the multiple choice
Cations in solution and their solvating water migrate towards cathode
Cations in solution and their solvating water migrate towards anode
Anions in solution and their solvating water migrate towards anode
Anions in solution and their solvating water migrate towards cathode
The cations attracted to capillary wall upon application of a potential, will move toward .. Multiple choice
Cathode while their solvating water migrate towards anode
Anode while their solvating water migrate towards cathode
Cathode and their solvating water migrate towards cathode
Anode and their solvating water migrate towards anode
The electro-osmotic flow is created inside the silica capillary as a result of ………… multiple choice
Depletion of the charge on capillary wall
Neutralization of the charge on the wall
Movements of the solvated cations toward cathode
The osmotic pressure
The electroosmotic flow is …………… the electrophoretic mobility multiple choice
Similar to
Ten times higher than
One tenth of
The first ions leave the capillary electrophoresis is …. Multiple choice
Smallest with positive charge
Largest with positive charge
Smallest with negative charge
Largest with positive charge
The last ions leave the capillary electrophoresis is …. Multiple choice
Smallest with positive charge
Largest with positive charge
Smallest with negative charge
Largest with positive charge
In caplillary electrophoresis, due to the electrophoretic mobility smaller anions move…….. Multiple choice
faster than larger anions toward cathode
Faster than larger anions toward anode
Slower than larger anions toward cathode
Slower than larger anions toward anode
In caplillary zone electrophoresis, neutral molecules ……………… multiple choice
Will move with EOF toward cathode
With larger size move faster toward cathode
Will move with EOF toward anode
With smaller size move faster toward cathodes
In capillary zone electrophoresis…………. Multiple choice
Cations move toward cathode and anions toward anode
Cations move toward anode and anions toward cathode
Both cations and anions move toward cathode
Both cations and anions move toward anode
The EOF ……………. The pH of the solution multiple choice
Is not affected by
Increase by increasing
Increase by decreasing
The negative charge on the silanol groups multiple choice
Increases with increasing pH
Decrease with increasing pH
Is constant between a pH of 3 and 8.
The EOF ……………with increasing the buffer strength.
Decrease
Increased
Not affected
Solvent consumption in CEis ………… HPLC
very larger than
Very smaller than
Similar to
CE is currently ………….. HPLC.
Had the same robustness as
Much more robust than
Much less robust than
All of the followings are advantages of CE over HPLC except …………………
More efficient
Shorter analysis time
Cheaper columns
Higher sensitivity
Which of the followings is not an advantages of CE over HPLC …………………
More efficient
More robust
Shorter analysis time
All of the followings are disadvantages of CE compared to HPLC except ………………
Less robust
More expensive
Less sensitive
Which of the followings is a disadvantages of CE compared to HPLC ……………… multiple choice
Less sensitive
More expensive
Consume large volume of solvents
The capillary in CE should be filled with ……………. Multiple choice
An aqueous solution containing electrolyte
Organic solvent such as ethanol
Non aqueous solution containing buffer
Aqueous solution with high salt content
The two forces which are responsible for separation in CE are …….. Multiple choice
Capillary action and osmotic pressure
Osmotic pressure and electrophoretic mobility
Electroosmotic flow and electrophoretic mobility
Pressure and capillary action
The electrophoretic mobility (μ) of the substance increase by increasing …….. Multiple choice
The charge on the ion
The ion radius
Viscosity
CE is similar to other types of chromatography in that the……….. The greater its mobility multiple choice
Greater the charge on the ion
Larger the ion
More closely an ion approximates to a sphere
The driving force in capillary electrophoresis is …………. Multiple choice
High pressure
Capillary action
High voltage
Not present
The analysis in capillary electrophoresis is carried out in ………………. Multiple choice
Capillary gas column
Stainless column
Narrow glass tube
Cuvette
The pH of the solution can NOT affect the ……….. Of the analyte. Multiple choice
Molecular shape
Mobility
Ionization
pKa
Separation of two drugs having similar shape and size but with different pKa values, can be obtained bycontrolling ……………… multiple choice
pH of the medium
The viscosity of the solution
The voltage
The buffer strength
For the analysis of basic drugs,the biggest numerical difference in mobility is obtained when multiple choice
PH
PH
PH
PH
If two basic drugs possess different pKa, ……….. mobility(s) at full charge ionization. Multiple choice
The drug with lower pka will has higher
They will have the same
The drug with higher pka will has higher
The compound that has the lowest mobility at pH 9.55 in capillary electrophoresis is ….. Multiple choice
Doxepin (pKa
Imipramine (pKa
Desimipramine (pKa
The faster compound at pH 9.55 in capillary electrophoresis is ………………..
Doxepin (pKa
Imipramine (pKa
Desimipramine (pKa
Which of the following drugs (codeine pka 8.2 and morphine pka 7.9) will have the highest mobility? Multiple choice
Codeine at pH
Codeine at pH
Morphine at pH
At pH 4.0 - 9.0 the silanol groups on glass caplillary wallmultiple choice
Bear a negative charge
Have a positive charge
Is neural
The amount of negative charge on the silica capillary wall is dependent on………….. Multiple choice
The applied voltage
Concentration of the analyte
PH of the medium
The charge on the uncoated silica capillary is neutralized by ………. Multiple choice
Anions in the running buffer
Cations in the running buffer
Sodium inside silica
Analyte ions
When a potential is applied across the uncoated silica capillary the multiple choice
Cations in solution and their solvating water migrate towards cathode
Cations in solution and their solvating water migrate towards anode
Anions in solution and their solvating water migrate towards anode
Anions in solution and their solvating water migrate towards cathode
The cations attracted to capillary wall upon application of a potential, will move toward .. Multiple choice
Cathode while their solvating water migrate towards anode
Anode while their solvating water migrate towards cathode
Cathode and their solvating water migrate towards cathode
Anode and their solvating water migrate towards anode
The electro-osmotic flow is created inside the silica capillary as a result of ………… multiple choice
Depletion of the charge on capillary wall
Neutralization of the charge on the wall
Movements of the solvated cations toward cathode
The osmotic pressure
The electroosmotic flow is …………… the electrophoretic mobility
Similar to
Ten times higher than
One tenth of
The first ions leave the capillary electrophoresis is ….
Smallest with positive charge
Largest with positive charge
Smallest with negative charge
Largest with positive charge
The last ions leave the capillary electrophoresis is …. Multiple choice
Smallest with positive charge
Largest with positive charge
Smallest with negative charge
Largest with positive charge
In caplillary electrophoresis, due to the electrophoretic mobility smaller anions move…….. Multiple choice
faster than larger anions toward cathode
Faster than larger anions toward anode
Slower than larger anions toward cathode
Slower than larger anions toward anode
In caplillary zone electrophoresis, neutral molecules ……………… multiple choice
Will move with EOF toward cathode
With larger size move faster toward cathode
Will move with EOF toward anode
With smaller size move faster toward cathodes
In capillary zone electrophoresis………….
Cations move toward cathode and anions toward anode
Cations move toward anode and anions toward cathode
Both cations and anions move toward cathode
Both cations and anions move toward anode
The EOF ……………. The pH of the solution
Is not affected by
Increase by increasing
Increase by decreasing
The negative charge on the silanol groups
Increases with increasing pH
Decrease with increasing pH
Is constant between a pH of 3 and 8.
The EOF ……………with increasing the buffer strength.
Decrease
Increased
Not affected
The force that makes the solution moves inside the capillary in CE is …….
Pressure
Siphoning
Electroosmotic flow
electrophoretic migration
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