Forelæsnings quiz

Bacteria regulate their gene expresion

In response to changes their growth enviroment

So that they do not exhaust the nutrients

Because there would sufficient space in the cell

Helix - turn - helix motifis are commonly found in

DNA bindings proteins

Autoiducers

RNA polymerases

An operon usually consists of

Promoter and terminator sequences

Inhibitor and structural genes

Promotor, operator and structural genes

Human genome contains:

Approximately 3 234 bases in the genome

Approximately 20 000-25 000 human protein-coding genes

Approximately 100 000 human protein-coding genes

Southern blotting technique is used to determine:

If a particular base sequence is present in a given DNA sample

If a particular base sequence is present in a given RNA sample

If a particular base sequence is present in a given protein sample

Bacterial plasmids can be used for

De novo synthesis of RNA

Insertion and replication of DNA fragments

Creation of mobile genetic elements

Complementary DNA is

DNA complementary to an mRNA sequence

DNA created from complementary introns and exons

DNA created using ribosomal RNA as template

Sanger dideoxy DNA sequencing method is based on

Addition of synthetic DNA linker to the DNA fragment

Controlled termination of replication by implementation of ddNTP’s

High temperature stabile DNA polymerases

Restriction enzymes cleave both strands of the duplex DNA at characteristic place which is

Restriction digest DNA fragments

Fingerprint of a DNA molecule

Specific palindromic sequences of four to eight base pairs

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