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Quizzes > Science

Glycolysis Pathway Quiz: Master the 10 Steps and Enzymes

Quick, free glycolysis steps quiz with instant results and explanations.

Editorial: Review CompletedCreated By: Into KustannusUpdated Aug 24, 2025
Difficulty: Moderate
2-5mins
Learning OutcomesCheat Sheet
Paper art illustration of glycolysis pathway quiz challenge on golden yellow background featuring enzymes and intermediates

Use this glycolysis pathway quiz to check each step from glucose to pyruvate and build speed with enzymes, intermediates, and ATP counts. To broaden your pathway knowledge, try the citric acid cycle quiz, review fundamentals with a biochemistry quiz, or compare pathways in a quick tca cycle quiz before taking another run.

Which cellular compartment houses the entire glycolytic pathway?
Peroxisome
Cytosol
Endoplasmic reticulum lumen
Mitochondrial matrix
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What is the net ATP yield per molecule of glucose converted to pyruvate via anaerobic glycolysis?
1 ATP
2 ATP
4 ATP
3 ATP
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Hexokinase phosphorylates glucose to produce which intermediate?
Fructose-6-phosphate
Glucose-1-phosphate
Fructose-1,6-bisphosphate
Glucose-6-phosphate
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Which enzyme converts glucose-6-phosphate to fructose-6-phosphate?
Phosphoglucose isomerase
Triose phosphate isomerase
Phosphoglycerate isomerase
Aldolase
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Which enzyme cleaves fructose-1,6-bisphosphate into dihydroxyacetone phosphate and glyceraldehyde-3-phosphate?
Pyruvate kinase
Aldolase
Phosphoglycerate mutase
Enolase
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The primary purpose of lactate dehydrogenase in anaerobic glycolysis is to:
Regenerate NAD+ for glyceraldehyde-3-phosphate dehydrogenase
Oxidize FADH2 to FAD
Generate acetyl-CoA for the TCA cycle
Produce ATP directly from pyruvate
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Which step is considered the rate-limiting step of glycolysis?
Dihydroxyacetone phosphate to glyceraldehyde-3-phosphate
Fructose-6-phosphate to fructose-1,6-bisphosphate
Phosphoenolpyruvate to pyruvate
Glucose to glucose-6-phosphate
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Which enzyme catalyzes the first committed step of glycolysis?
Phosphofructokinase-1 (PFK-1)
Phosphoglucose isomerase
Pyruvate kinase
Hexokinase
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How many ATP are invested during the energy investment phase of glycolysis per glucose?
2
1
4
3
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Which enzyme interconverts dihydroxyacetone phosphate and glyceraldehyde-3-phosphate?
Triose phosphate isomerase
Phosphoglycerate mutase
Enolase
Phosphoglucose isomerase
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NADH is produced in glycolysis at which step?
Phosphofructokinase-1
Pyruvate kinase
Glyceraldehyde-3-phosphate dehydrogenase
Hexokinase
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Which high-energy intermediate is formed directly by enolase?
3-phosphoglycerate
Phosphoenolpyruvate (PEP)
2-phosphoglycerate
1,3-bisphosphoglycerate
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ATP acts on PFK-1 primarily as a(n):
Competitive inhibitor at the fructose-6-phosphate site
Allosteric inhibitor
Substrate that increases Vmax without regulation
Covalent activator
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Which pair lists both enzymes that generate ATP by substrate-level phosphorylation in glycolysis?
Phosphoglycerate kinase and pyruvate kinase
Hexokinase and PFK-1
GAPDH and triose phosphate isomerase
Aldolase and enolase
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In red blood cells, diversion through the Rapoport-Luebering shunt does which of the following?
Produces NADPH for antioxidant defense
Converts lactate back to pyruvate aerobically
Increases net ATP yield of glycolysis
Generates 2,3-BPG at the expense of ATP production
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A deficiency of pyruvate kinase in erythrocytes most directly leads to which clinical outcome?
Leukopenia due to impaired DNA synthesis
Methemoglobinemia due to excess NADH
Polycythemia due to increased 2,3-BPG
Hemolytic anemia due to decreased ATP
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Arsenate toxicity disrupts glycolysis by uncoupling ATP formation from which step?
Hexokinase
Enolase
Glyceraldehyde-3-phosphate dehydrogenase
PFK-1
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Compared to glucokinase, hexokinase typically has which kinetic property for glucose?
Higher Km (lower affinity)
Zero-order kinetics at low glucose
Lower Km (higher affinity)
No product inhibition by G6P
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Glyceraldehyde-3-phosphate is phosphorylated using inorganic phosphate, not ATP, during its conversion to 1,3-bisphosphoglycerate.
False
True
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Which cofactor requirement is correct for pyruvate kinase catalytic activity?
Requires K+ and Mg2+ for optimal activity
Requires biotin as a carboxylation cofactor
Requires thiamine pyrophosphate
Requires FAD as an electron acceptor
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Study Outcomes

  1. Recall Glycolysis Steps -

    List the ten sequential reactions in glycolysis from glucose to pyruvate to reinforce your pathway memory.

  2. Identify Enzymatic Catalysts -

    Recognize and name the key enzymes that catalyze each step of the glycolysis pathway.

  3. Distinguish Energy Phases -

    Differentiate between the ATP investment and ATP generation phases to understand energy flow in glycolysis.

  4. Map Metabolic Intermediates -

    Match each glycolytic intermediate to its corresponding reaction, enhancing your recall of molecular structures.

  5. Self-Assess Pathway Mastery -

    Use the scored glycolysis quiz to evaluate your understanding and identify areas for review.

Cheat Sheet

  1. Net Reaction and Energy Yield -

    By the end of glycolysis one glucose molecule is converted into two pyruvate with a net gain of two ATP and two NADH (overall equation: C6H12O6 + 2 ADP + 2 Pi + 2 NAD+ → 2 C3H3O3− + 2 ATP + 2 NADH + 2 H+ + 2 H2O). Mastering this stoichiometry is a staple of any glycolysis quiz and helps you quickly balance energy budgets in metabolic problems.

  2. Investment vs. Payoff Phases -

    Glycolysis begins with a preparatory phase that consumes two ATP to phosphorylate glucose and split it into two triose phosphates, then shifts to a payoff phase producing four ATP and two NADH. Remember "2 in, 4 out" to swiftly answer questions on energy flow in the glycolysis pathway quiz.

  3. Key Regulatory Enzymes -

    Hexokinase (or glucokinase in liver), phosphofructokinase-1 (PFK-1), and pyruvate kinase serve as irreversible, rate-limiting steps subject to allosteric control by ATP, AMP, and fructose-2,6-bisphosphate. Knowing these checkpoints and their effectors is crucial for tackling regulation questions on a glycolysis steps quiz.

  4. Enzyme Order Mnemonic -

    Use "Goodness Gracious, Father Franklin Did Go By Picking Pumpkins (to) Prepare Pies" to recall hexokinase, phosphoglucose isomerase, PFK-1, aldolase, triose phosphate isomerase, glyceraldehyde-3-phosphate dehydrogenase, phosphoglycerate kinase, phosphoglycerate mutase, enolase, and pyruvate kinase. A strong mnemonic saves time when listing enzymes under quiz conditions!

  5. Physiological and Clinical Relevance -

    Beyond energy metabolism, glycolytic flux influences redox balance via NADH production and is hijacked in the Warburg effect by cancer cells for rapid growth. Linking these concepts to real-world examples boosts your recall and demonstrates pathway significance in a medical or biochemistry quiz context.

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