3020 E2

Which general transcription factor serves as a bridge between the transcription factor that recognizes the TATA box and RNA Polymerase II?

Consider an artificial transcription factor consisting of the DNA binding domain of the lac repressor attached to the transcription activation domain of GaI4. How would this artificial transcription factor behave in yeast?

It would repress transcription from synthetic promoters that include the lac repressor binding site.

It would not work at all- prokaryotic DNA binding domains don't work in eukaryotes like yeast.

It would activate transcription from synthetic promoters that include the lac repressor binding site.

It would repress transcription from synthetic promoters that include the GaI4 activator binding site.

It would activate transcription from synthetic promoters that include the GaI4 activator binding site.

How does tryptophan affect the function of the trpR repressor?

Tryptophan binds to the repressor and prevents the repressor from binding DNA

Tryptophan binds to the repressor, enabling the repressor to bind to DNA

Tryptophan has no effect on the trpR repressor, it only affects attenuation

The trpR repressor is covalently modified by addition of tryptophan

The trpR repressor is released from an inhibiting protein in the presence of tryptophan

What is the function of TFIID?

Terminating transcription

Removing inhibitory proteins

Making a bridge between the transcription factor that recognizes the TATA box and RNA polymerase II

Phosphorylating the carboxy-terminal domain (CTD) of RNA polymerase

Binding to the TATA box

One type of transcription termination in prokaryotes is caused by

Supercoiling the DNA template

RNA polymerase passing a polyadenylation signal in the DNA template

Formation of a cloverleaf structure in the RNA transcript

Formation of a hairpin loop followed by a string of Us in the RNA transcript

Cleavage by sigma

Which of the following is not a common DNA binding domain?

Which of the following is not a mechanism of eukaryotic transcriptional repression?

Making contact with mediator

Masking the activation domain of a transcriptional activator

Competing with a transcriptional activator for DNA binding

Inhibiting DNA looping or bending

All of these are mechanisms of transcriptional repression

If the two tryptophan codons in the trpL leader peptide coding region were replaced by codons for valine, how would the operon respond?

Attenuation would not happen

Attenuation would always occur

Attenuation would happen when tryptophan levels are high, while transcription would continue when tryptophan levels are low

Attenuation would happen when valine levels are high, while transcription would continue when valine levels are low

Attenuation would be affected both by tryptophan levels and valine levels

RNA polymerase are similar to DNA polymerases in that: (Pick true)

Both enzymes copy all the DNA in the cell

Both enzymes synthesize nucleic acids in 5' to 3' direction only

Both enzymes require a primer

Both enzymes use the same nucleotide triphosphates as substrates

Mark which are true for the Carboxy Terminal Domain of RNA polymerase II

It is phosphorylated by Mediater

It is phosphorylated by TFIIH

Its phosphates are removed when transcription elongation begins

Splicing factors bind when it is phosphorylated

Acetylation of it increases transcription

Indicate which statements are true for prokaryotes

Chromatin remodeling affects rates of transcription

A single RNA polymerase produces all of the transcripts required by the cell

Transcription initiation can be affected by transcriptional repressors and activators

Multiple general transcriptional factors are required to create a transcription initiation complex

RNA polymerase are multi-subunit enzymes

Indicate which statements are true for eukaryotes

Chromatin remodeling affects rates of transcription

A single RNA polymerase produces all of the transcripts required by the cell

Transcription initiation can be affected by transcriptional repressors and activators

Multiple general transcriptional factors are required to create a transcription initiation complex

RNA polymerase are multi-subunit enzymes

What chromatin modifications increase transcription?

Removal of histone octomers

Methylation of DNA at CpG islands

Histone acetylation

Histone methylation

Which transcription factors are controlled by unmasking (release from an inhibitory protein)?

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3020 E2

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