Video training

How many sperm cells should be put into the Cap and Non-Cap tubes?

3x106

300x106 .

10x106

To recover the sperm pellet from the density gradient the following steps are acceptable:

A pasture pipette can be passed through the seminal plasma and enhance column to remove the sperm pellet from the bottom of the conical tube.

A wide orifice transfer pipette can be used to remove the seminal plasma from the enhance gradient and another wide orifice transfer pipette can be used to remove the sperm pellet from the bottom of the conical tube.

large gauge hypodermic needle attached to a syringe can be passed through the seminal plasma and enhance gradient to remove the sperm pellet from the bottom of the conical tube.

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