Aseptic Technique
Aseptic Technique Quiz
Test your knowledge of aseptic techniques essential for maintaining sterility in laboratory environments. This quiz will help you identify contaminants, proper procedures, and best practices when working with biological materials.
Key areas covered include:
- Sources of contamination
- Handling equipment safely
- Understanding sterilization techniques
- Identifying different types of contamination
The main issue with keeping extra items in the hood that these items:
Personalize your work space
Block airflow and are a source of contamination
Can get damaged when sprayed with ethanol
There are some differences with what is done in the video and what we do (different organization’s rules). Which of the following do we require?
Hand washing
Hair net
Face mask
Removal of jewelry
Answers a and d
Answers a and b
How did they sterilize the glass petri dish?
Autoclave only
Foil and autoclave
Ethanol
Ethanol and fire from bunsen burner
Where are gloves worn?
Fume hoods when working with hazardous chemicals
Biological hoods when working with bacteria
Laminar hoods when doing plant tissue culture
Answer a and b
All of the above
The purpose of the laminar flow is:
Provide a clean work area
Keep your tissues from drying out
Provide relaxing background noise
Adjust the humidity in the air
How should you move within the hood while making tissue transfers?
Fast, quick movements
Very slow
Smooth
Where should you not spray ethanol?
Floor of hood
Back of hood
Sides of hood
Outside hood
Answers b and d
Answers c and d
What is not sprayed and wiped with 70% ethanol?
Microscope
Bags that contain petri dishes
Scalpel
Media bottles before pouring
In the hood, where do the scalpel and forceps stay when not in use?
Petri dish
95% ethanol
In media of the tissue you were working with
70% ethanol
How can you tell if you have fungus or bacterial contamination?
One is colorful while the other just white
Bacteria is fuzzy
Bacteria is slimy
Fungus is slimy
Airflow can (which one does not apply)?
Dry out your tissues
Can cause swirls that pick up contaminants in the room and blow them on to your sample
Can pick up contaminants in the hood and blow them on to your sample
Can blow contaminants from your hands and clothes on to your sample
Filter fumes out of the air
All of the above
None of the above
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