Lab methods
Lab Methods Mastery Quiz
Test your knowledge of laboratory methods with our engaging quiz! This quiz features a range of questions on techniques like PCR, gel electrophoresis, and more.
Whether you're a student, teacher, or research enthusiast, you'll find this quiz helpful in understanding key lab concepts. Prepare to challenge yourself with:
- Multiple choice questions
- Technical terminology
- Real-world applications
Which of the following statements about polymerase chain reaction (PCR) is FALSE?
PCR uses a DNA polymerase from a thermophilic bacterium.
PCR is particularly powerful because after each cycle of replication, there is a linear increase in the amount of DNA available.
For PCR, every round of replication is preceded by the denaturation of the double-stranded DNA molecules.
Which of the following techniques is not appropriate if you want to examine whether a gene is expressed in a specific tissue?
RNA-Seq
In situ hybridization
Immunohistochemistry
Microarray
Gel electrophoresis separates proteins on the basis of which two factor? (All correct answers are required for the points)
The protein's cell of origin
The protein's size
The protein's net charge
The protein's affinity for a ligand molecule
Which of the following terms describes a mouse in which a particular gene has been genetically modified?
Transgenic mouse
Replicated mouse
Transfected mouse
Hybrid mouse
Analyzing the expression of thousands of genes simultaneously can be done using which of the following techniques? (multiple alternatives)
ELISA
Northern blot
DNA microarrays
Next-generation sequencing (RNASeq)
Western blot
A microtome is used to
Slice thin sections (paraffin-embedded) of specimens
View microscopic organisms
Dissect cellular organelles
Focus short wavelengths of light
Which method is used to study detect the presence of proteins in a sample?
Next generation sequencing
Quantitative-PCR
Southern blotting
Western blotting
PCR uses a DNA polymerase from a thermophilic bacterium.
True
False
PCR is particularly powerful because after each cycle of replication, there is a linear increase in the amount of DNA available.
True
False
For PCR, every round of replication is preceded by the denaturation of the double-stranded DNA molecules.
False
True
The PCR will generate a pool of double-stranded DNA molecules, most of which will have DNA from primers at the 5′ ends.
True
False
Flow cytometry is based upon the use of _____________
Immunological techniques
Radioactive elements
Energy content
Heavy isotopes
DNA sequencing refers to the
Technique used to determine the phosphate sequence in a DNA molecular
Technique used to determine the base sequence in a DNA molecule
All of these
Technique used to determine the sugar sequence in a DNA molecule
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