Genetics Final

If a double stranded DNA fragment contains 30% guanine, what is the thymine content?
70%
30%
20%
40%
60%
Which of the following is the correct sequence of cycles would you expect to see in a standard PCR?
Denaturation-Extension-Annealing
Annealing-Denaturation-Extension
Denaturation-Extension-Denaturation
Denaturation-Annealing-Extension
Extension-Annealing-Denaturation
In the Hershey-Chase experiment, what was radioactively labeled by growing bacteriophage in 32Phosphorus containing medium?
Ghost phage particles
Proteins
Lipids
DNA
Carbohydrates
Which of the following enzyme fills the gap let on the lagging strands of ends of chromosomes during DNA replication
Reverse Transcriptase
Telomerase
Topoisomerase
Helicase
Primase
In the Meselson-Stahl experiment, if DNA replication was semiconservative, what were the different fractions of DNA molecules observed in the final generation (G2)?
50% 14N/14N & 50% 15N/15N
100% 14N/14N
100% 15N/14N
50% 14N/14N & 50% 14N/15N
50% 15N/14N & 50% 15N/14N
In deoxyribonucleic acid, hydroxyl (OH) group is attached to what carbon of the sugar
1'
2'
3'
4'
5'
Nucleotides are made up of...
Define Genetics
DNA in each human cell is made up of approximately how many DNA nucleotides?
1 billion
500 million
2 billion
3 billion
100 million
DNA methylation of promoter sequences of a gene leads to
Gene activation
Gene repression
Both activation and repression
Deletion of the promoter
Nothing happens
EcoRI restriction enzyme recognizes 6 bp nucleotide sequences. iIf the occurrence of each nucleotide is random, what is the expected frequency of EcoRI recognition sites in a genome?
16
64
256
1024
4096
Basal transcriptional factors bind to which of the following DNA sequences in eukaryotes?
CAT box
TATA box
GCGCGGG
GC box
CACAAT
Which of the following sites is where a foreign gene is inserted into a plasmid vector during the creation of a recombinant DNA molecule?
Replication orgin site
Multiple cloning site
Antibiotic resistant gene site
Lac Z gene site
B and D only
Name an example of regulatory DNA sequences.
Transcriptional activator
Basal transcriptional factors
Silencer
Co-repressor
Transcriptional repressor
Acetyl marks are added to which of the following amino acids in histone H3 protein?
Methionine
Glycine
Arginine
Lysine
All of the above
Transposable elements make up what percent of the human genome?
80%
60%
35%
45%
1.5%
In Gen1, the frequency of 2pq in a population is 0.07. If the population undergoes inbreeding, what will be the frequency of 2pq in Gen4
0.35
0.175
0.0875
0.7
1
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