Hema Moving Exam

Reticulocytes are composed of?

Refers to immaturely released reticulocytes during increased RBC production

Give one example of supravital stain

Retics count normal value for adults

Retics count normal value for infants

Are counted in the larger square

ABSOLUTE RETICULOCYTE COUNT REFERENCE RANGE

Correction factor for 40-45% Hct value?

Correction factor for 25-34% Hct value?

INEFFECTIVE ERTYTHROPOIESIS

EFFECTIVE ERYTHROPOIESIS

Most convenient and commonly used method for making peripheral blood films

Size of drop of blood in making blood smears

Angle of spreader slide:

Fixative used in blood smears?

WBC’s are counted in consecutive fields as the blood smear is moved from side to side

LONGITUDINAL METHOD

CROSS-SECTIONAL OR CRENELLATION METHOD

EXAGGERATED BATTLEMENT METHOD

Use to assess adrenocortical function

Shift to the left:

Presence of immature granulocytes

Increased hypersegmented neutrophils

Normal Value of Lymphocytes

Hydrodynamic Focusing and Impedance

Impedance and optical scatter

In histogram, x-axis represents?

RBC Histogram: Cause of bimodal curve

RBC Histogram: Cause of widened curve

Best used for the differentiation of WBC

Most common source of error in hematology machines

(Thick smear/Thin smear): Decreased Pressure

(Excessively Blue stain/Excessively Pink stain): Too high alkalinity of the stain or diluent

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