Exercise 4
Mastering Sterile Techniques Quiz
Test your knowledge on sterile techniques and their importance in microbiology with our engaging quiz! This quiz covers key concepts related to avoiding contamination, the significance of aseptic methods, and proper procedures for culturing microbes.
Enhance your understanding with questions that include:
- The definition of sterile technique
- Reasons for practicing sterile methods
- Features of pure cultures and incubation processes
Why is sterile technique important? Give two reasons.
to prevent contamination of your culture with organisms from the environment
to prevent the culture from contaminating you or others. In this exercise, you
to sterile techniques and procedures used to ensure a safe laboratory.
to do it in such a way that you will not permit any organism in the environment from entering the tubes and plates.
To store cultures under conditions suitable for growth
Free of contamination
Sterilization
Disinfection
Aseptic
Sterile
This refers to sterile techniques and procedures used to ensure a safe laboratory.
Incubate technique
Aseptic technique
Pure culture
Sterile technique
Store cultures under conditions suitable for growth, often in an incubator.
Sterile
Aseptic
Incubate
Autoclave
Heat stable
Free of viable bacteria or viruses.
Sterile
Aseptic
Autoclave
Pure culture
Disinfected
You will inoculate known microbes into your
Autoclave
Sterile tubes
Sterile plates
Nutrient agar
Sterile nutrient broth tubes
Sterile nutrient agar plates
Before studying the characteristics of any one of the species in the laboratory, it is often necessary to isolate that species as a
Why should you incubate your agar plate’s bottom side up?
There is also risk of cross contamination between different specimens.
to lessen contamination risks from airborne particles landing on them
To produce isolated colonies of an organism (primarily bacteria) on an agar plate.
A culture in which only one strain of bacteria is present
Why is it important to avoid digging the loop into the agar?
The bacteria can grow in the gauge preventing the bacteria from growing into distinct colonies.
To prevent the accumulation of water condensation that could disturb or compromise a culture.
To obtain isolated, pure colonies
A culture in which only one strain of bacteria is present
What is the purpose of streak plate?
The bacteria can grow in the gauge preventing the bacteria from growing into distinct colonies.
To obtain isolated colonies from an inoculum by creating areas of increasing dilution on a single plate.
to lessen contamination risks from airborne particles landing on them
A culture in which only one strain of bacteria is present
What is the definition of a pure culture?
A culture in which only one strain of bacteria is present
As they allow for the study of one species without the worry of contamination from other organisms.
There is also risk of cross contamination between different specimens.
Condensation will interfere with colonial growth and may introduce a contaminant
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