Sanger's Sequencing Method
Test Your Knowledge on Sanger's Sequencing Method
Welcome to the Sanger's Sequencing Method quiz! This quiz is designed to help you test your understanding of one of the foundational techniques in molecular biology. Dive into questions that cover the principles, reagents, and techniques used in DNA sequencing.
Prepare to challenge yourself with:
- Multiple-choice questions
- Structure and fun
ction of nucleotides - Principles of gel electrophoresis
What is the full form of ddNTP?
Deoxyribonucleotide triphosphate
Dideoxynucleoside triphospate
Dideoxyribonucleotide triphosphate
Deoxydiribonucleoside triphosphate
What is the most unique feature of the structure of ddATP?
It contains cytosine as a nulceotide
It does not contain O atom at 3' prime end
It does not contain O atom at 5' prime end
It contains adenine as a nucleotide
What is the first step of Sanger's DNA sequencing method after adding all reagents?
Providing heat for denaturing template DNA.
Reducing temperature for DNA synthesis
Denaturing DNA polymerase
Adding more ddNTP at high concentration
Which reagent is essential for termination of DNA synthesis
DNTP
DdNTP
DNA polymerase
Radiolabelled primer
On what prinicple does gel electrophoresis separate molecules
Solubility of molecule
Density of molecule
Volume of molecule
Mass/size of molecule
How is the DNA sequence read after electrophoresis
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