Sanger's Sequencing Method

Create an educational illustration depicting the Sanger sequencing process, including DNA strands, ddNTPs, electrophoresis gel, and a laboratory setting with scientists conducting experiments.

Test Your Knowledge on Sanger's Sequencing Method

Welcome to the Sanger's Sequencing Method quiz! This quiz is designed to help you test your understanding of one of the foundational techniques in molecular biology. Dive into questions that cover the principles, reagents, and techniques used in DNA sequencing.

Prepare to challenge yourself with:

Multiple-choice questions
Structure and function of nucleotides
Principles of gel electrophoresis

9 Questions2 MinutesCreated by SequencingStar502

What is the full form of ddNTP?

Deoxyribonucleotide triphosphate

Dideoxynucleoside triphospate

Dideoxyribonucleotide triphosphate

Deoxydiribonucleoside triphosphate

What is the most unique feature of the structure of ddATP?

It contains cytosine as a nulceotide

It does not contain O atom at 3' prime end

It does not contain O atom at 5' prime end

It contains adenine as a nucleotide

What is the first step of Sanger's DNA sequencing method after adding all reagents?

Providing heat for denaturing template DNA.

Reducing temperature for DNA synthesis

Denaturing DNA polymerase

Adding more ddNTP at high concentration

On what prinicple does gel electrophoresis separate molecules

Solubility of molecule

Density of molecule

Volume of molecule

Mass/size of molecule

Sanger's Sequencing Method

Test Your Knowledge on Sanger's Sequencing Method

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