MTAP Microbiology 1 and 2 Part 2
Media used for isolation of Aspergillus
Czapek agar
Niger seed agar
Cornmeal agar
Urea agar
It is used for the detection of C. neoformans
Czapek agar
Cornmeal agar
Staib's
Casein medium
Used to differentiate T. Rubrum from T. mentagrophytes = I. Urea agar II. Rice medium III. Potato dextrose agar IV. Potato flake agar
I and III
I and IV
II and III
II and IV
All are criteria for virus classification EXCEPT
Presence of envelope
Capsid morphology
Site of virion
Diameter
All are TRUE about viruses EXCEPT
Specimen must be collected during acute phase of the infection
Swab must be made with dacron and nylon but calcium alginate could also be used if the two are not available
Storage temp is 4 deg C
Transport media is maintained at pH 7.2
Storage temp for transporting viruses when there will be a delay of 6 days or longer
4 - 6 deg C
2 - 8 deg C
- 120 deg C
- 20 deg C
Storage temperature for transporting viruses within 3 days
1-4 deg C
-20 deg C
2-8 deg C
-70 deg C
All are INCORRECT about viruses EXCEPT
Transport media can be frozen
Presence of antibiotics in transport media
Transport media is maintaine at 6.2 pH
Neutral red is used as indicator
Viral transport media is maintained at what pH
6.2
6.5
7.2
7.4
Anticoagulant used when PCR is requested
Gray top
Purple top
Pearl top
Royal blue top
Anticoagulant that inhibits DNA polymerase, thus, not to be used in nucleic acid testing
Purple top
Green top
Pearl top
Light blue top
Inclusion bodies found in Yellow fever virus
Torres council man bodies
Negri bodies
Guameri paschen bodies
Bollinger bodies
Usually found in urine specimen
VSV
CMV
HSV
RSV
All are usually found in specimens with respiratory syndromes EXCEPT
Togavirus
RSV
Influenzae virus
Parainfluenzae virus
Usually found in stool specimen
Togavirus
Rotavirus
HSV
CMV
Type of cell culture where Hep-2 and HeLa are used
Primary cell culture
Diploid cell culture
CPE
Heteroploidy
It shows swelling and clumping "grape-like cluster"
Alpha herpes viruses
Picornaviruses
Papilomavirus
Adenovirus
It shows pyknosis
Adenovirus
Paramyxovirus
Picornavirus
Papilomavirus
It shows formation of fused cells
Paramyxovirus
Papilomavirus
Paliomavirus
Picornavirus
It produces a narrow, diffuse zone of Beta-hemolysis close to the colony
S. pyogenes
S. agalactiae
S. pneumoniae
S. epidermidis
It produces colonies that are creamy, white with a dull surface, and described as colonies with feet or pedicles
Staphylococci
Streptococci
C. albicans
Fungi
Organism that produces a bull's eye colony
Proteus spp.
B. pertussis
S. agalactiae
S. pyogenes
Produces colonies resembling bread crumbs
Neisseria
Nocardia
S. aureus
Streptococci
Has old sock odor
P. aeruginosa
P. mirabilis
S. aureus
Haemophilus spp.
Has putrid odor
P. mirabilis
P. aeruginosa
S. aureus
Nocardia spp.
It forms a small, "fuzzy-edged" colony with an umbonate center on SBA or CHOC agar
S. marcescens
B. cereus
E. corrodens
S. agalactiae
Swarming colonies is a characteristic of which genus of bacteria?
Streptococci
Serratia
Neisseria
Proteus
PH indicator used in OF media
Phenol red
Neutral red
Bromthymol blue
Methylene blue
All are fermentable carbohydrates in TSI EXCEPT
Lactose
Sucrose
Glucose
Maltose
Percentage of peptone in OFBM
1%
2%
0.1%
0.2%
Useful in the identification of PPM
Decarboxylase test
Malonate test
Urease test
DNase test
PH indicator used in LIA
Bromcresol purple
Bromcresol blue
Phenol red
Neutral red
Putrescine is an alkaline amine product of which bacterial enzyme?
Arginine
Lysine
Ornithine
Phenylalanine
Positive result of decarboxylase test
Yellow
Red
Purple
Green
Added to MAC to identify E. coli 0157:H7
Mannitol
Sorbitol
Fructose
Lactose
Reagent used in catalase test
Phenol red
Hydrogen peroxide
Rabbit's plasma
Bromthymol blue
All are automated EXCEPT
API
Vitek 2
TREK
WalkAway
When cleansing the skin with alcohol and then iodine for the collection of a blood culture, the iodine (or iodophor) should remain intact on the skin for at least
10 seconds
30 seconds
60 seconds
5 minutes
Semisolid transport media, such as Amies, Stuart, or Cary-Blair, are suitable for the transport of swabs for culture of most pathogenic, EXCEPT
Neisseria gonorrhoeae
Enterobacteriaceae
Campylobacter fetus
Streptococcus pneumoniae
CNA agar is used primarily for the recovery of?
Neisseria spp
Enterobacteriaceae
P. aeruginosa
S. aureus
In the United States, most blood agar plates are prepared with 5% or 10% RBCs obtained from:
Sheep
Horse
Humans
Dogs
CCFA is used for the recovery of
Y. enterolitica
Y. intermedia
C. perfringens
C. difficile
Specimens for virus culture should be transported in media containing
Antibiotics and 5% sheep blood
Saline and 5% sheep blood
22% bovine albumin
Antibiotics and protein nutrient
The most sensitive method for the detection of Beta-Lactamase in bacteria is by the use of
Chromogenic cephalosporin
Penicillin
Oxidase
Chloramphenicol acetyltransferase
All are under macrolides EXCEPT
Azithromycin
Eythromycin
Imipenem
Clarithromycin
The VP test detects which product of glucose fermentation?
Acetoin
Nitrite
Acetic acid
Hydrogen peroxide
At which pH does the MR test become positive?
7.0
6.5
6.0
4.5
Care must be taken when identifying biochemical isolates of Shigella because serological cross reactions occur with
E. coli
Salmonella spp.
Pseudomonas spp.
Proteus spp.
The most noted difference between P. Aeruginosa and S. Maltophilia are
Oxidase, Catalase, and TSI
Oxidase, catalase, and ONPG
Oxidase, 42 deg C growth, and polar tuff of flagella
Catalase, TSI, and Pigment
Cetrimide agar is used as a selective isolation agar for which organism?
Acinetobacter spp.
P. aeruginosa
Moraxella spp.
S. maltophilia
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