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Lecture 4 (DYES ONLY)

What color does PAS show?
Bright Red
Pale Blue
Orange
Magenta
Which of these are PAS positive?
Mucin of Goblet Cells
Basement Membrane
Brush Border cells of GIT
Kidneys
Glycogen
Cartilages
Nucleus
All but nucleus
Used to stain support tissue CT:
PAS
Masson's Trichrome
Giemsa Stain
Nissl Stain
What colors does Masson's Trichrome give?
Red, blue, yellow
Red, blue, green
Green, yellow, red
Orange, yellow, blue
What appears as blue in Masson's Trichrome?
Nucleus
Cytoplasm
RBCs
Collagen
What appears as red in Masson's Trichrome?
Nucleus
Cytoplasm
RBCs
Collagen
What appears as green in Masson's Trichrome?
Nucleus
Cytoplasm
RBCs
Collagen
What appears as bright red in Masson's trichrome?
Nucleus
Cytoplasm/Muscle/RBCs/Keratin
Ribosomes
Collagen
What colors are in Van gieson?
Red, blue, yellow
Red, blue, green
Green, Orange, Red
Green, Orange, Blue
Which of these is for CT stain?
PAS
Silver & Gold
Van Gieson
Nissl Stain
What appears as red in Van Gieson?
Nucleus
Cytoplasm/RBCs
Ribosomes
Collagen
What appears as blue in Van Gieson?
Nucleus
Cytoplasm/RBCs
Ribosomes
Collagen
What appears as yellow in Van Gieson?
Nucleus
Ribosomes
Collagen
Cytoplasm and RBCs
Which of these is for CT stain & Special Epithelium Stain?
PAS
Silver & Gold
Azan
Nissl Stain
What appears as bright red in Azan?
Nucleus
Mucin
RBC
Collagen
What appears as blue in Azan?
Nucleus
Mucin
RBCs
Collagen
What appears as orange in Azan?
Nucleus
Mucin
RBCs
Collagen
Which of these is for standard blood cells smear and bone marrow?
Giemsa Stain
Silver & Gold
Azan
Nissl Stain
What appears as blue in Giemsa Stain?
Nucleus
Cytoplasm
RBCs
Collagen
What appears as pale blue in Giemsa Stain?
Nucleus
Cytoplasm
RBCs
Collagen
What appears as pink in Giemsa Stain?
Nucleus
Cytoplasm
RBCs
Collagen
Which of these is used for nervous tissue studies?
PAS
Silver & Gold
Van Gieson
Nissl Stain
Which of these is used to demonstorate rER of neurons?
PAS
Silver & Gold
Azan
Nissl Stain
Magenta means
Bright red color staining of carbohydrates by PAS.
Bright pink color staining of RBC by Giemsa Stain
Bright red color of muscle and cytoplasm by Masson's Trichrome
Bright red color of nuclei by Azan Stain
The stain we use to study connective tissue which gives blue/green color to Collagen is
Masson's Trichrome
Giemsa Stain
Hematoxylin and Eosin
Silver and Gold
Which of the following is best visualized by silver stain?
Collagen
Nucleuolus
Nervous Tissue
Fibrocytes
Regarding collagen, all the following are correct except:
The orientation of tropocollagen gives it its birefingent appearance.
Collagen fibers are arranged in bundles in tissues
Takes red/pink color with Eosin
Takes red color with Masson's Trichrome
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