Gram Stain
Gram Stain Quiz
Test your knowledge of the Gram staining process, a fundamental technique in microbiology used to differentiate bacterial species based on the characteristics of their cell walls. This quiz covers essential components and procedures involved in Gram staining.
Features of the quiz:
- Multiple choice questions
- Topics include primary stains, mordants, and more
- Ideal for students and educators
What is the primary stain?
Iodine
Methyl Crystal Violet
Carbol fuchsin
Alcohol
Safranin
What is the Mordant?
Alcohol
Iodine
Carbol fuchsin
Methyl Crystal Violet
Safranin
What is the decolourizing agent?
Iodine
Methyl Crystal Violet
Alcohol
Safranin
Carbol fuchsin
What is the couterstains?
Carbol fuchsin
Methyl Crystal Violet
Iodine
Safranin
Alcohol
What type of staining is gram stain?
Differential staining
Simple Staining
Acid Fast Staining
Endospore Staining
What type of complex is formed with the primary stain and the mordant?
CV-I complex
I-CV complex
CI-V complex
V-IC complex
V-CI complex
Why does gram positive retain the staining after the decolourizing agent, but not gram negative?
Gram positive has a thicker peptidoglycan layer
Gram positive has a thiner peptidoglycan layer
Gram negative has a thicker peptidoglycan layer
Gram negative has no peptidoglycan layer
Gram positive has no peptidoglycan layer
How long is the primary stain kept for after flooding?
30 seconds
10 seconds
5 seconds
3 seconds
20 seconds
8 seconds
How long is the mordant kept for after flooding?
30 seconds
10 seconds
5 seconds
3 seconds
20 seconds
8 seconds
How long is the decolourizing agent kept for after flooding?
30 seconds
10 seconds
5 seconds
3 seconds
20 seconds
8 seconds
How long is the counterstain kept for after flooding?
30 seconds
10 seconds
5 seconds
3 seconds
20 seconds
8 seconds
How long is the loop allowed to cool after heating?
30-40 seconds
10-20 seconds
5-10 seconds
3-5 seconds
20-30 seconds
8-10 seconds
How many layers does gram positive have?
3
2
1
5
4
How many layers does gram negative have?
3
2
1
5
4
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