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A detailed illustration of various types of microtomes and histopathological techniques, showcasing tools like rotary microtome, rocking microtome, and specimen preparation methods in a well-organized lab setting.

Histopathology Lab Quiz

Test your knowledge in histopathology with our comprehensive quiz designed for laboratory professionals and students. This quiz covers a wide range of topics, including microtomy, fixation techniques, and tissue processing.

Challenge yourself and improve your understanding of histopathology while enjoying the quiz experience!

50 questions to test your knowledge
Multiple choice format
Learn about microtomes, fixatives, and more

50 Questions12 MinutesCreated by CuttingEdge42

Rocking microtome was invented by?

Trefall

Minot

Adams

Quackett

Rotary microtome was invented by

Quackett

Trefall

Adams

Minot

Sliding microtome was invented by

Minot

Adams

Trefall

Quackett

Microtome is a process by which tissue is trimmed and cut into uniformly thin slices or sections.

True

False

Invented by Quackett in 1848. Used to cut undehydrated tissues in a frozen state.

Ultrathin microtome

Rotary microtome

Rocking microtome

Freezing microtome

What kind of microtome cuts a serial section of LARGE BLOCKS of paraffin embedded tissue

Rotary microtome

Sliding microtome

Rocking microtome

Ultrathin microtome

Identify the given microtome knife; both side are straight, and is used for cutting hard or tough specimen:

Plane-wedge knife

Biconcave knife

Plane concave knife

None of the above

Tissue is held in position

Knife carrier

Knife

Block holder

Ratchet feed wheel

What microtome knife is concave, and at the same time flat?

Plane concave

Biconcave

Plane-wedge knife

Process whereby the burr formed during honing is removed

Stropping

Honing

Tissue specimen is immersed in a watch glass containing isotonic solution

Teasing

Crushing

Streaking

Pull apart

Process whereby small pieces of tissue not more than 1mm

Pull apart

Crushing

Touch prep

Spreading

Selected portion is transferred to a clean slide

Streaking

Spreading

Crushing

Touch prep

The following belongs to the fundamentals of specimen dissection EXCEPT:

Orient

Measure

Sample

Labelling

The following information should be present in requisition form EXCEPT:

Patient ID

Type of specimen

Additional notations

None of the above

What is the right order of tissue processing?

Fixation, Dehydration, Embedding, Clearing, Infiltration, Trimming, Section cutting, Staining, Mounting, Labeling

Fixation, Dehydration, Clearing, Infiltration, Embedding, Trimming, Section cutting, Staining, Mounting, Labeling

Dehydration, Embedding, Clearing, Infiltration, Trimming, Section cutting, Staining, Mounting, Labeling, Fixation

Clearing, Dehydration, Fixation, Infiltration, Embedding, Trimming, Section cutting, Staining, Mounting, Labeling

All of the following are nuclear fixatives EXCEPT:

Carnoy's fluid

Bouin's fluid

Heidenhain's Susa

Orth's fluid

The most widely used fixative is:

10%

30%

20%

70%

Decalcification is being done on some specimens to prevent:

Cutting of hard tissues

Excess fluids in processing

Tissues from becoming brittle

All of the above

Formol-Saline fixative is made up of the following components EXCEPT:

NaCl

Formaldehyde

Distilled water

Ethyl alcohol

What fixatives are recommended for glycogen fixation

Formaldehyde

Neutral buffered saline

Formol-saline

Alcoholic fixatives

Formol-corrosive fixative is a combination of:

Formaldehyde and HCl

Formaldehyde and Mercuric chloride

Formaldehyde and disodium hydrogen phosphate

Formaldehyde and picric acid

The most common and fastest decalcifying agent:

EDTA

Formic acid

Trichloroacetic acid

Nitric acid

Perenyi's fluid is composed of the following EXCEPT:

Hydrochloric acid

Nitric acid

Chromic acid

Absolute ethyl alcohol

A fixative recommended for fixing chromosomes, lymph gland and urgent biopsies:

Carnoy's fluid

Regard's fluid

Heidenhein's susa

Picroformol fixative

A specimen submitted in the laboratory was not placed in a fixative. What is most likely to occur in the tissue sample?

A. Putrefaction

B. A only

C. Autolysis

D. A and C

Which of the following enhances the fixation of tissues?

Agitation

Cold temperature

Presence of mucus

Presence of fat

All of the following can be used for decalcification EXCEPT:

Chelating agent

Alcohol

Electrophoresis

Ion exchange resins

The most common chelating agent used as a decalcifying agent:

EDTA

Fluoride

Hydrochloric acid

Von Ebner's fluid

The process of removing excess fixative from tissues after fixation to improve staining and remove artifacts from the tissue is known as:

Dezenkerization

Secondary fixation

Post chromatization

Washing out

Acetone as fixative:

Recommended for the study of water diffusible enzymes

Recommended for fixing fats

Preserves glycogen well

None of the above

True about secondary fixation:

Helps ensure further hardening and preservation of tissues

Removes excess fixative

Remove pigment artifact such as the brown pigments caused by the formalin

Enhances nuclear staining

The breakdown of tissue by bacterial action is called:

Autolysis

Putrefaction

Oxidation

Denaturation

The fixation of tissue begins at the:

Center and proceeds outward

Periphery and proceeds inward

Center and proceeds inward

Periphery and proceeds outward

A good fixative is one that:

Makes tissue more permeable to fluids

Preserves tissues in an un-life-like manner

Does not harden tissue quickly

Enhances putrefaction of tissue components

Ethanol is the most commonly used dehydrating agent:

True

False

Dehydration is done in increasing concentration of the dehydrating agent:

True

False

Xylene is the most commonly used agent in clearing:

True

False

What are the roles of fixation?

To preserve the tissue

To prevent breakdown of cellular elements

To coagulate or precipitate protoplasmic substances

All of the above

Tissue must be place in a fixative slowly after removal from the body

True

False

The chemical constituent of the fixative is taken in and becomes part of the tissue by forming cross-links and giving stability to the protein

Additive fixation

Non-additive fixation

None of the above

All of the above

Practical considerations of fixation includes:

Speed, Penetration, Volume, Duration of fixation

Speed, Time, Penetration, Volume

Speed, Time, Volume, Duration

Penetration, Speed, Time, Volume

Effects of fixatives includes:

Harden and softens friable tissues

Make cells resistant to damage and distortion

They inhibit bacterial decomposition

All of the above

The following are the characteristics of a good fixative EXCEPT:

Safe to handle

Cheap

Stable

Inhibits virus decomposition

What range of percentage do we need to wash out excess amount of picric acid in Bouin's solution?

60-80%

50-70%

30-40%

40-50%

A process whereby positively charged calcium ions are attracted to a negative electrode and subsequently remove from the decalcifying solution

Electrophoresis

Ion exchange resin

Chelating agents

None of the above

The commonly used clearing agents for dealcoholization in the embedding process are the following EXCEPT:

Xylene

Chloroform

Benzene

Cedarwood

Designed to mount water-miscible preparations directly from water:

Aqueous mounting media

Resinous mounting media

What type of blocking-out mold consists of 2 L-shaped strips of heavy brass or metal arranged on a flat metal plate which can be moved to adjust the block to tissue size?

Plastic embedding rings & base mold

Leuckhart's embedding mold

Compound embedding unit

None of the above

This is recommended for urgent biopsies for delicate tissues such as lung, brain, connective tissues, decalcified bones, eyes, spleen & CNS.

Vacuum Embedding

Celloidin

Plastic

Paraffin

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