RNA Isolation Quiz 1
RNA Isolation Knowledge Check
Test your understanding of RNA isolation techniques with our engaging quiz! This quiz covers essential aspects of RNA isolation procedures, from spin protocols to troubleshooting common issues. Perfect for laboratory professionals and students looking to sharpen their knowledge.
Join us in exploring the intricacies of RNA isolation:
- Multiple choice questions
- Instant feedback on your answers
- Enhance your laboratory skills
What is a limiting reagent for Bioanalyzers?
Conditioning Solution
Marker
Filtered Gel
Gel-Dye Mix
What speed do you spin filtered gel? And how many microliters do you filter through the column?
1500g; 550mL
1500g; 550uL
1500g; 500mL
1500g; 500uL
How would you dilute a previously normalized sample to run a RIN?
3uL 1XTE + 2uL sample
3uL 1xTE +1uL sample and heat denature
2uL 1XTE + 2uL sample
3uL 1XTE + 2uL sample and heat denature
What concentration do we speedvac samples to?
5ug/uL
5 ng/uL
3 pg/uL
5 pg/uL
When do you spin at program 4?
After 80% etOH is added
I dunno
Never
Ask me later
The CLS you're working with hands you this and you have 3 trays, what spin step are you on?
RW1; Program 3
RPE; Program 3
80% EtOH; Program 4
Rebind; Program 3
What's wrong with this? Hint: You have 2 batches
Nothing
I dunno
It's fine
Missing batch number
What are the settings of program 4 on the RNA isolation centrifuges?
2:00; 10000g
2:00; 1000g
2:00; 100g
2:00; 20817g
When do you run program 5?
After 80% EtOH
After program 4 columns are spun
During the lunar eclipse
When Mecury is in retrograde
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