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Experiments (before Rosen et al’s publication) showing that that PPARγ can induce adipogenesis and cause transdifferentiation show that PPARγ is ____ to induce adipogenesis?
Necessary
Sufficient
Cell Dependent
Cool Enough
This paper wanted to show that PPARγ is ______ to induce adipogenesis?
Necessary
Sufficient
Irrelevant
Acting as a mitogen
Why didn’t the authors just create a homozygous null mutant?
It felt like a lot of work and they didn’t have the time/energy
The confounding effects of PPARγ knockdown meant that the embryos died before forming adipose tissue
Previous efforts had failed to make an actual homozygous null mutant
It had already been done and the authors were using mouse chimeras to confirm the results
What is a mouse chimera?
A mouse with the head of a goat and the tail of a snake
A single organism composed of cells of different genotypes
A single organism composed of tissues made of the same genotype but from different species
A single organism in which the zygote has been targeted via a target vector to change its genotype
In class we’ve talked about different ways to make knockout cells. Which of the following methods did the authors use in this paper to generate their chimera?
A targeting vector that uses homologous recombination through Cre/Lox
CRISPR/Cas9 introduced by a vector
RNAi introduced by a vector
A targeting vector that uses homologous recombination through FRT/FLP sites
Wishful thinking
What are Southern blots used for?
Detection of specific proteins
Detection of specific DNA sequences
Detection of specific mRNA sequences
Finding the nearest chicken and biscuit joint.
Why did the authors look into the gene dosage effect of PPARγ?
With the time they saved on not making a homozygous null mutant, they finally had time for this experiment
It confirms that PPARγ is sufficient for adipogenesis
It confirms the results from Figures 2 and 3 in vitro
It shows if PPARγ is necessary for early adipose cell determination, subsequent lipogenesis or both
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