PROTEIN PURIFICATION

Protein Purification Quiz

Test your knowledge on protein purification techniques with our engaging quiz designed for both students and professionals. Dive into the world of biochemistry and explore the methods used to isolate and analyze proteins.

By participating in this quiz, you will:

Enhance your understanding of protein purification methods.
Challenge your knowledge with targeted questions.
Discover new insights in the field of biochemistry.

10 Questions2 MinutesCreated by AnalyzingAtom42

How does dialysis purify a protein sample?

Using a semi-permeable bag containing the proteins, smaller molecules are diffused into a buffer solution

Using a semi-permeable bag containing the proteins, proteins diffuse out of the bag leaving the impurities

Using a semi-permeable bag containing the proteins, the buffer solution dials the number of the impurities

Using a semi-permeable bag containing the proteins, the buffer solution purifies the bag leaving only the proteins.

What type of column chromatography uses size to purify a protein sample?

Gel Filtration

Anion Exchange

Affinity Chromatography

SDS-PAGE Chromatography

Why is gel filtration not as effective for purifying protein samples?

Proteins generally have similar sizes

Columns are always short and therefore ineffective.

Proteins have interactions with the gel used in gel filtration.

Proteins are large and will therefore get stuck inside the column.

How does SDS denaturation help with electrophoresis?

It converts proteins to have the same charge to mass ratio.

It converts proteins to have the same shape

It converts proteins to have the same volume

It converts proteins to have the same moral values

What purpose does polyacrylamide gel in SDS-PAGE serve?

It acts as a sieve in which proteins will travel in

It acts as a stabilizer to guide proteins to travel in a straight line

It helps proteins not interact with other proteins

All choices are correct

What is the end product of the Edman Sequencing called?

Phenylthiohydantoin

Phenylisothiocyanate

Phenylthioisohydanate

Phenylthiormamtoin

How is mass spectroscopy used in identifying the protein?

Identifying through fingerprinting using spectral database

Analyzing numerical mass data to identify each amino acid for sequencing

All of the choices are correct

None of the choices are correct

What does Extinction coefficient signify?

He different measures of absorption of light in a medium

The different measures of reflection of light in a medium

The rate of denaturation of a protein in a medium

The amount of determination a substance has to exist

Why is it important for the protein to be crystallized in X-ray crystallography?

It is important for the protein to have an ordered structure to capture a clear picture of the protein

It is important for the protein to be see through to be able to produce a diffraction pattern

It is important for the protein solid and tough to diffract X-ray

It is important that the protein be shiny to diffract X-ray

PROTEIN PURIFICATION

Protein Purification Quiz

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