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Order of Draw Game: Practice the Correct Tube Sequence

Quick, free order of draw practice test. Instant feedback.

Editorial: Review CompletedCreated By: Timothee MontiasUpdated Aug 24, 2025
Difficulty: Moderate
Grade: Grade 12
Study OutcomesCheat Sheet
Paper art themed trivia quiz about The Order of Draw Challenge for nursing and phlebotomy students.

This order of draw quiz helps you practice the correct tube sequence and remember it under pressure. Answer 20 quick questions with instant feedback to find weak spots and build confidence for labs or exams. For more prep, try the phlebotomy multiple choice quiz, the free phlebotomy practice test, or review phlebotomy test questions.

What is the correct first tube in the standard venipuncture order of draw to minimize contamination risk?
Red serum tube
Light blue sodium citrate tube
Blood culture bottle or sterile yellow SPS tube
Gray fluoride oxalate tube
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Which tube is drawn immediately after blood cultures in the standard venipuncture order of draw?
Red serum
Lavender EDTA
Green heparin
Light blue sodium citrate
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After light blue, which category of tube is next in the order of draw for venipuncture?
Gray fluoride oxalate tubes
Heparin tubes (green)
EDTA tubes (lavender or pink)
Serum tubes (red, gold, or orange)
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Following serum tubes, which additive category comes next in the standard order of draw?
EDTA (lavender)
Sodium citrate (light blue)
Heparin (green)
Fluoride oxalate (gray)
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In the standard order of draw, which tube is drawn just before the gray fluoride oxalate tube?
Green heparin
Red serum
Light blue sodium citrate
Lavender EDTA
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Which tube color and additive pair is correctly matched for its position after serum tubes?
Green tube with heparin
Lavender tube with EDTA
Light blue tube with sodium citrate
Gray tube with sodium fluoride
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The gray tube is placed last in the venipuncture order of draw to prevent carryover of which additive?
Sodium fluoride and potassium oxalate
Lithium heparin
EDTA (K2 or K3)
Sodium citrate
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Blood culture tubes are collected first primarily to avoid contamination by which source?
Light exposure
Air exposure
Skin antiseptics or additives from other tubes
Hemolysis from tight tourniquets
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Drawing a lavender EDTA tube before a serum tube can falsely alter which analyte most directly due to carryover?
Calcium
Cholesterol
Creatinine
Bilirubin
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Using a winged infusion set for coagulation testing may require what additional step before the light blue tube?
Delay tourniquet release until after filling
Draw a gray tube first
Invert the light blue tube 20 times
Use a discard tube to fill the tubing dead space
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What is the rationale for drawing the light blue sodium citrate tube before serum tubes?
To minimize hemolysis in serum
To avoid contamination with clot activators that can affect coagulation tests
To prevent fluoride inhibition of enzymes
Because citrate needs more time to clot
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Which statement describes the correct handling of royal blue trace element tubes in relation to order of draw?
Draw royal blue before blood cultures
Always draw royal blue first
Draw royal blue after gray tubes
Draw royal blue with no additive after serum tubes; draw EDTA-labeled royal blue with EDTA group
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In capillary collections, what is the correct order of draw?
Other additive tubes, blood gases, serum, EDTA
Serum, EDTA, blood gases, other additive tubes
Blood gases, EDTA, other additive tubes, then serum
EDTA, serum, blood gases, other additive tubes
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Which additive carryover from a green tube can most directly affect coagulation tests if order is violated?
Heparin
Fluoride
Oxalate
Silica clot activator
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The presence of clot activator in a serum separator tube can falsely shorten clotting times if it contaminates which tube?
Gray fluoride oxalate
Light blue sodium citrate
Green heparin
Lavender EDTA
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For trace metal analysis, why should a royal blue no-additive tube be drawn after serum tubes?
Because serum tubes are always sterile
To allow EDTA to stabilize metals first
To ensure fluoride preserves metals
To avoid carryover of anticoagulants that could chelate trace metals
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For arterial blood gas syringes plus venous tubes, which statement maintains correct sequencing?
Mix ABG with EDTA and proceed
Collect ABG separately using arterial technique; venipuncture order remains unchanged
Draw ABG after gray tube
Draw ABG into a green tube first, then others
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Which specific test is most susceptible to heparin contamination if order is not followed?
Albumin
Serum iron
Total protein
Thrombin time
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When collecting both coagulation and trace element EDTA tests with a winged set, what sequence is correct?
Serum, royal blue EDTA, light blue
Royal blue EDTA, discard, light blue, serum
Discard, serum, light blue, royal blue EDTA
Discard tube, light blue, serum/others, royal blue EDTA with EDTA group
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True or false: For point-of-care coagulation devices using capillary blood, venous order of draw rules do not apply to that capillary collection.
True
False
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Study Outcomes

  1. Understand the rationale behind the proper sequence for blood sample collection.
  2. Identify the correct order of draw for different laboratory tests.
  3. Apply best practices to maintain sample integrity during phlebotomy.
  4. Analyze common errors in blood sample collection and their potential effects.
  5. Evaluate clinical scenarios to determine appropriate blood collection methods.

Order of Draw Quiz Review Cheat Sheet

  1. Understanding the order of draw - Knowing the correct sequence for tube collection is your first line of defense against contaminated samples and false lab results. Think of it like following a recipe: skip a step and the whole batch could be ruined! Master this, and you'll be a lab ninja in no time.
  2. Standard order of draw - Follow the classic lineup: blood culture, sodium citrate (light blue), serum (red or gold), heparin (green), EDTA (lavender), then glycolytic inhibitors (gray). Each tube has a special role, and jumping around can mix additives in ways that wreck your test data. Stick to the script to keep results rock-solid.
  3. Use mnemonic devices - Memory tricks like "Young Bodies Really Should Get Large Grains" can turn a daunting list into a catchy jingle. When you belt it out, each tube color and order sticks in your head like a favorite tune. Before you know it, you're recalling the sequence faster than you can say "phlebotomy."
  4. Know your additives - From sodium citrate in light blue tubes for coagulation to EDTA in lavender for hematology, every tube's chemistry matters. Mix the wrong additive into your sample and you could throw off entire panels of blood work. Get to know what's inside each tube, and you'll never mix up your tests again.
  5. Proper tube inversion - Gentle mixing is key - think "no shake, just roll." For example, sodium citrate tubes need about 3 - 4 gentle inversions to blend blood and additive without forming clots. Master the swirl and you'll avoid hemolysis or clotting mishaps that can invalidate results.
  6. Minimize additive carryover - Skipping or reordering tubes can transfer tiny traces of chemicals that throw off sensitive assays. Imagine sugar in your salt - it just doesn't work! By staying disciplined, you'll slash the risk of cross-contamination and keep your data pristine.
  7. Follow CLSI guidelines - The Clinical and Laboratory Standards Institute sets the gold standard for phlebotomy practices. These rules are designed to harmonize procedures worldwide, so labs everywhere speak the same "tube language." Trust the experts and your draw technique will be globally recognized.
  8. Blood culture tubes first - Drawing these before anything else ensures microbial cultures aren't tainted by other additives. It's like putting on fresh gloves before handling a sterile tray. Start strong to nail your microbiology analysis every time.
  9. Serum tubes without additives - Plain red-top tubes come before clot-activator tubes to avoid accidental clotting inside the sample. This simple order tweak preserves the purity of serum you need for many chemistry tests. Keep unadulterated serum samples on your A-game checklist.
  10. Practice makes perfect - Regularly reviewing and rehearsing the draw sequence cements your skills under pressure. Turn each venipuncture into a mini training session, and pretty soon you'll breeze through complex panels without blinking. Confidence grows with every successful stick!
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