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Quizzes > Health & Medicine

Phlebotomy Quiz: Test Your Certification Knowledge

Quick, free quiz with phlebotomy test questions. Instant results.

Editorial: Review CompletedCreated By: Khalid UmarUpdated Aug 27, 2025
Difficulty: Moderate
2-5mins
Learning OutcomesCheat Sheet
Paper art illustration of a clipboard blood drop syringe and exam sheet on dark blue background for phlebotomy quiz

This phlebotomy quiz helps you practice core skills and exam topics so you can check your knowledge before certification. Work through realistic items on order of draw, tube colors, and patient ID with quick feedback. For more study, try a full length phlebotomy practice test, focus on a phlebotomy order of draw quiz, or review a phlebotomy multiple choice quiz for chapter-style questions.

Which additive is in a light-blue top tube used for coagulation testing?
EDTA
Sodium fluoride/potassium oxalate
Sodium citrate
Lithium heparin
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During venipuncture, the needle bevel should face up and the insertion angle should be approximately
45 to 60 degrees
35 to 45 degrees
15 to 30 degrees
5 to 10 degrees
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Maximum recommended tourniquet time to minimize hemoconcentration is
3 minutes
1 minute
30 seconds
2 minutes
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Which site is contraindicated for venipuncture on a patient with a mastectomy on the right side?
Dorsal hand veins on the left
Right arm
Left forearm
Left antecubital fossa
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What is the maximum puncture depth for a heel stick on a newborn?
1.0 mm
2.5 mm
2.0 mm
3.0 mm
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Serum differs from plasma because serum
Is collected in a green top tube
Contains anticoagulant
Lacks fibrinogen after clotting
Is obtained without centrifugation
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Which test requires a patient to be in a fasting basal state?
Random cortisol
PT/INR
CBC
Fasting plasma glucose
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In the CLSI recommended order of draw using ETS, which tube is collected first?
Green heparin
Light-blue citrate
Serum tube (red/gold)
Blood culture bottles or yellow SPS
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Which action best prevents hemolysis in a potassium specimen drawn with EDTA?
Gentle inversion 8 to 10 times, avoid vigorous shaking
Draw the tube first in the order
Use a 20-gauge needle for all draws
Allow the tube to stand uncapped for 30 minutes
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For blood cultures collected with a winged infusion set, which bottle should be filled first?
Serum separator tube
Anaerobic
EDTA tube
Aerobic
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Which anticoagulant prevents clotting by binding calcium?
Thrombin
Heparin
Gel separator
EDTA and sodium citrate
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Before a glucose tolerance test, the patient should typically
Increase carbohydrate restriction for 1 week
Maintain normal diet and activity for 3 days, then fast 8 to 12 hours
Fast for 24 hours and avoid water
Take a laxative the night before
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What essential identifiers must be on a patient specimen label at minimum?
Patient initials and room number
Patient full name and unique second identifier (e.g., DOB or MRN), collection date/time, collector ID
Patient name only
Provider name and test name only
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Capillary collection order of draw is
EDTA, serum, blood gases, other additives
Blood gases, EDTA, other additive tubes, serum
Serum, blood gases, EDTA, other additives
Other additives, EDTA, blood gases, serum
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How many gentle inversions are recommended for a light-blue sodium citrate tube?
8 to 10 inversions
No inversion required
5 to 8 inversions
3 to 4 inversions
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Petechiae appearing below the tourniquet during a draw most likely indicate
Arterial puncture
Capillary wall fragility or coagulation issues
Venous spasm
Allergic reaction to gloves
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Cold agglutinin specimens should be
Frozen until analysis
Placed on ice immediately
Exposed to light to reduce activity
Kept warm at 37 C from collection to testing
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When using a butterfly for coagulation testing only, what should be done first?
Draw EDTA first
Fill the light-blue tube directly without a discard
Release the tourniquet after needle removal
Draw and discard a small, nonadditive tube to clear air in the tubing
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What is the proper order for removing PPE after a blood draw to minimize contamination?
Gloves, mask, hand hygiene, gown, eye protection
Gown, mask, gloves, eye protection, hand hygiene
Mask, eye protection, gown, gloves, hand hygiene
Gloves, gown, eye protection, mask, hand hygiene
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Which temperature handling is appropriate for a cryofibrinogen specimen?
Chill immediately on ice
Room temperature for 24 hours
Freeze whole blood immediately
Keep at 37 C until clotting and separation
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Study Outcomes

  1. Apply Venipuncture Techniques -

    Demonstrate proper needle selection, site preparation, and order of draw to perform accurate blood collection.

  2. Identify Specimen Tubes and Additives -

    Recognize common tube colors, their additives, and corresponding tests to ensure correct sample usage.

  3. Analyze Patient Safety Protocols -

    Evaluate patient identification steps, infection control measures, and handling procedures to maintain safety and compliance.

  4. Interpret Exam-Style Questions -

    Break down CPT phlebotomy practice test items to understand question formats and key terminology.

  5. Evaluate Specimen Handling Procedures -

    Assess correct labeling, transportation, and storage methods to prevent pre-analytical errors.

  6. Enhance Exam Confidence and Readiness -

    Build self-assurance by applying real-world scenarios and targeted practice questions before the certification exam.

Cheat Sheet

  1. Order of Draw Mnemonic -

    Mastering the CLSI-recommended tube sequence (blood culture, light blue, red, gold/SST, green, lavender, gray) is crucial for accurate results. Try the classic mnemonic "Stop Light Red, Go Green, Lavender Gray" to lock it into memory. This tip often appears on phlebotomy certification practice quiz questions to test your knowledge of additive interference.

  2. Antiseptic Technique & Site Selection -

    Proper cleansing with 70% isopropyl alcohol and allowing 30 seconds of drying time prevents hemolysis and contamination. Selecting the antecubital fossa or dorsal hand veins, while avoiding scars and hematomas, is a key skill tested on a CPT phlebotomy practice test. Following CDC and WHO guidelines ensures both patient safety and sample integrity.

  3. Vein Anatomy & Needle Gauge -

    Familiarize yourself with major veins (median cubital, cephalic, basilic) and choose gauges 21 - 23 for most adults, 23 - 25 for pediatric draws. Understanding vein depth and size reduces the chance of hematoma and patient discomfort - topics frequently featured in phlebotomy exam prep questions. Use diagrams from accredited lab manuals to reinforce spatial awareness.

  4. Preventing Preanalytical Errors -

    Gently invert anticoagulant tubes 5 - 10 times immediately after collection to mix additives thoroughly and prevent clotting. Label each specimen at the bedside with two identifiers within two minutes, per CLSI H3 guidelines, to avoid misidentification. Questions on a phlebotomy technician certification quiz often focus on these critical preanalytical steps.

  5. Patient Identification & Safety -

    Always verify two patient identifiers (name and DOB) against the requisition before puncture to comply with Joint Commission standards. Adhering to universal precautions - gloves, hand hygiene, proper sharps disposal - minimizes occupational exposure risks. Solid mastery of these protocols builds confidence for both the CPT phlebotomy practice test and real-world clinical settings.

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