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Quizzes > Health & Medicine

ALAT certification test practice quiz

Quick, free ALAT practice test with instant results and clear explanations.

Editorial: Review CompletedCreated By: Jamilah MacarampatUpdated Aug 26, 2025
Difficulty: Moderate
2-5mins
Learning OutcomesCheat Sheet
Paper art illustration for ALAT practice test quiz on golden yellow background

This quiz helps you check your ALAT certification test knowledge in lab animal care and protocols. Work through realistic questions, get instant feedback with clear explanations, and try a timed round while you track your score. If you want more targeted drills, take our ALAT practice test, or build broader test skills with a NOCTI practice test.

What does PPE stand for in a laboratory animal facility?
Personal Protective Equipment
Protective Personnel Equipment
Personal Performance Equipment
Physical Protection Equipment
PPE stands for Personal Protective Equipment and is used to protect personnel from biological, chemical, and physical hazards during animal care. Proper PPE reduces the risk of contamination and injury. Common items include gloves, gowns, masks, and eye protection.
What is the ideal ambient temperature range for most laboratory mice?
26-30°C
18-22°C
15-20°C
20-26°C
Most laboratory mouse strains thrive at an ambient temperature between 20°C and 26°C. Temperatures outside this range can cause stress, affecting their physiology and research outcomes. Proper HVAC control ensures consistent environmental conditions.
Which organization accredits laboratory animal care programs on a voluntary basis?
FDA
USDA
CDC
AAALAC International
AAALAC International provides voluntary accreditation for animal care and use programs via peer review. Accreditation demonstrates commitment to high standards beyond regulatory compliance. Many institutions seek AAALAC to show excellence in animal welfare.
Under the Animal Welfare Act, which federal agency regulates laboratory animal use in the US?
NIH
EPA
FDA
USDA
The United States Department of Agriculture enforces the Animal Welfare Act, ensuring standards for animal care in research and exhibition. The USDA Animal and Plant Health Inspection Service conducts inspections and handles compliance. Regulations apply to certain warm-blooded species.
How often should general animal housing areas be cleaned and disinfected?
Monthly
Weekly
Biannually
Daily
Daily cleaning and disinfection of animal housing areas are recommended to control odors and pathogens. Regular sanitation reduces the risk of disease transmission and maintains animal welfare. Detailed SOPs define specific tasks and disinfectant contact times.
Which sign indicates dehydration in a rodent?
Polyphagia
Skin tenting
Increased activity
Excessive grooming
Skin tenting, where skin remains elevated after being pinched, indicates dehydration in rodents. Other signs include sunken eyes and reduced skin elasticity. Prompt fluid replacement is essential.
What essential information must appear on a rodent cage label?
Building address
Animal ID and protocol number
Staff initials only
Food brand
Cage labels must include animal ID and IACUC protocol number for traceability and compliance. They also often include date, sex, and strain. Accurate labels support proper care and recordkeeping.
What does the biohazard symbol warn of?
Radioactive materials
Infectious agents hazard
Electrical hazard
Chemical hazard
The biohazard symbol denotes the presence of biohazardous materials such as infectious agents. It warns personnel to use appropriate containment and PPE. Proper labeling prevents accidental exposures.
What is a fomite in animal facility context?
Animal handler
Inanimate object that transmits disease
Disease-causing microbe
Airborne particle
A fomite is an inanimate object that can carry infectious agents between animals or from animals to handlers. Examples include cages, bedding, and equipment. Disinfection of fomites is key to infection control.
What is the maximum recommended oral gavage volume for a 25 g mouse?
0.5 mL
1 mL
0.25 mL
2 mL
The maximum safe oral gavage volume for a 25 g mouse is 0.25 mL to avoid risk of aspiration or esophageal injury. Volumes should not exceed 10 mL/kg. Proper technique helps prevent complications.
What is the normal respiratory rate of a resting mouse?
20-30 breaths per minute
300-400 breaths per minute
90-230 breaths per minute
400-500 breaths per minute
A resting mouse breathes at approximately 90-230 breaths per minute. Rates outside this range can indicate distress or disease. Monitoring respiration helps assess animal health and anesthesia depth.
Which route is commonly used for subcutaneous injections in rodents?
Intramuscular
Tail vein
Intracardiac
Loose skin over the scruff
The scruff, or loose skin over the neck and back, is the preferred site for subcutaneous injections in rodents due to minimal risk of hitting vital structures. Volume should be limited. Proper restraint and technique ensure animal welfare.
Which bedding material is generally recommended for rodent cages?
Cedar shavings
Corncob or paper-based
Straw
Pine shavings
Corncob and paper-based beddings are preferred for rodents due to low dust and absence of aromatic oils that can cause respiratory issues. Cedar and pine contain phenols that may be harmful. Bedding choice impacts animal comfort and health.
What is the typical quarantine period for newly received rodents?
4-6 weeks
1-2 weeks
8-10 days
3-4 days
Newly received rodents are commonly quarantined for 1-2 weeks to monitor health status and prevent pathogen introduction. During this time, health checks and sentinel testing occur. Length may vary by facility SOPs.
What is the purpose of sentinel animals in a rodent facility?
Breed for experiments
Serve as treatment controls
Provide enrichment
Monitor for pathogens
Sentinel animals are exposed to soiled bedding from colony cages to monitor for infectious pathogens across a facility. Regular serological testing of sentinels helps detect agents before widespread infection. This proactive approach maintains colony health.
Which inhalation anesthetic is most commonly used for rodent anesthesia in lab settings?
Halothane
Isoflurane
Ether
Chloroform
Isoflurane is widely used for rodent anesthesia due to its rapid induction and recovery, as well as minimal metabolic toxicity. It allows precise control of anesthetic depth. Other agents have greater side effects or safety concerns.
How do you calculate the drug dose for a 25 g mouse if the drug concentration is 1 mg/mL and the recommended dose is 5 mg/kg?
0.05 mL
0.125 mL
0.025 mL
0.5 mL
For a 25 g (0.025 kg) mouse, the dose of 5 mg/kg equals 0.125 mg. At 1 mg/mL concentration, the required volume is 0.125 mL. Accurate calculations are critical for efficacy and safety.
What method is appropriate for sterilizing surgical instruments?
Autoclaving
Manual detergent wash
Alcohol wipe
UV lamp
Autoclaving uses steam under pressure to kill all microorganisms and spores, making it the gold standard for surgical instrument sterilization. Detergent washes remove debris but do not sterilize. Alcohol and UV are disinfection methods but not reliable for complete sterilization.
What is the recommended relative humidity range for rodent rooms?
10-20%
70-90%
40-70%
80-100%
Maintaining relative humidity between 40% and 70% prevents respiratory issues and skin dryness in rodents and supports environmental stability. Extremes in humidity can lead to microbial growth or dehydration. HVAC systems monitor and control humidity levels.
Which sign suggests that a rabbit is experiencing pain?
Tooth grinding (bruxism)
Weight gain
Increased play
Excessive grooming
Tooth grinding, or bruxism, is a well-recognized indicator of pain in rabbits. Other signs include hunched posture, reduced appetite, and grinding of teeth. Early pain detection improves welfare and outcomes.
How should used needles and syringes be disposed?
Biohazard bag
Bottom of cage
Regular trash
Puncture-resistant sharps container
Sharps must be placed directly into puncture-resistant sharps containers to prevent needle-stick injuries and cross-contamination. Biohazard bags are for non-sharp contaminated waste. Compliance with OSHA standards is mandatory.
Which zoonotic condition caused by a fungus can be transmitted from rodents to humans through direct contact?
Leptospirosis
Ringworm
Toxoplasmosis
Tularemia
Ringworm, or dermatophytosis, is a fungal infection that can pass from rodents to humans via direct contact with infected animals or bedding. It causes a characteristic skin rash. Appropriate hygiene and PPE reduce risk.
Which member is required on an Institutional Animal Care and Use Committee (IACUC)?
A construction manager
A veterinarian with laboratory animal experience
A pharmaceutical representative
A legislator
Federal regulations mandate that an IACUC include a veterinarian experienced in laboratory animal medicine to ensure animal welfare and proper veterinary care. Other required members include a scientist and a non-affiliated community member.
According to the Animal Welfare Act, how long must animal use records be retained?
1 year
At least 3 years
6 months
10 years
The Animal Welfare Act requires institutions to retain records of animal use, including protocols and health records, for at least three years. This ensures traceability and compliance during USDA inspections.
Which method is considered acceptable for euthanasia of zebrafish?
CO2
Freezing
Decapitation without anesthesia
Overdose of tricaine methanesulfonate (MS-222)
An overdose of MS-222 is the AVMA-approved method for euthanizing zebrafish, providing rapid and humane loss of consciousness. Freezing and CO2 are considered inhumane for fish. Proper buffering and post-treatment ensure animal welfare.
What is the recommended air changes per hour for a rodent housing room?
30-35 ACH
20-25 ACH
2-4 ACH
10-15 ACH
Rodent housing rooms should have 10 - 15 air changes per hour to control temperature, humidity, and airborne contaminants. Adequate ventilation is crucial for animal health and research integrity.
When lifting heavy cages, how many people are recommended to assist?
One
Three
Four
Two
To prevent injury and ensure safe handling, two people should lift and move heavy animal cages or racks. Team lifting reduces strain and the risk of dropping cages. SOPs often define weight limits and lifting techniques.
What does a Body Condition Score (BCS) of 1 indicate in mice?
Emaciated
Over-conditioned
Ideal weight
Obese
A BCS of 1 signifies an emaciated animal with prominent bones and minimal muscle. Ideal BCS is usually around 3 on a 5-point scale. Regular scoring monitors health and nutrition.
Why should diet changes be introduced gradually over 7 days?
To save labor
To prevent digestive upset
To increase appetite
To test palatability
Gradual diet transitions over 7 days allow the gut microbiota and digestive enzymes to adjust, preventing diarrhea or anorexia. Sudden changes can stress the gastrointestinal tract. This practice supports animal welfare and study consistency.
Which environmental enrichment is suitable for laboratory rats?
Wire mesh
PVC tubes
Metal chains
Cedar blocks
PVC tubes provide hiding space and exploratory enrichment for rats, enhancing welfare and natural behaviors. Enrichments should be safe, non-toxic, and sanitized regularly. Inappropriate materials like cedar can be harmful.
What type of glove should be selected to protect against rodent allergens while maintaining dexterity?
Vinyl gloves
Powder-free nitrile gloves
Cotton gloves
Leather gloves
Powder-free nitrile gloves offer barrier protection against allergens and chemicals while providing dexterity needed for fine tasks. Latex can cause allergic reactions and vinyl offers less chemical resistance. Proper glove selection reduces occupational exposure.
Which biosafety level requires directional airflow and access through two self-closing doors?
BSL-4
BSL-1
BSL-3
BSL-2
BSL-3 laboratories require directional airflow, sealed entries, and two self-closing doors to contain pathogens transmitted via inhalation. This level protects personnel and the environment. BSL-4 adds more stringent measures with full-body suits.
Which condition must be minimized to grow obligate anaerobes in a culture?
Moisture
Oxygen
Temperature
Nutrients
Obligate anaerobes cannot tolerate oxygen and require strict anaerobic conditions for growth. Culture systems use reducing agents or anaerobic chambers to remove oxygen. Temperature, moisture, and nutrients are managed but are not inhibitory if oxygen is controlled.
According to the NIH guidelines, which biosafety level is required for work with transgenic rodents carrying a novel gene with unknown pathogenic potential?
BSL-1
BSL-4
BSL-3
BSL-2
NIH guidelines recommend at least BSL-2 containment for transgenic rodents with unknown pathogenic potential to protect personnel and prevent environmental release. BSL-1 is for well-characterized agents. Higher levels depend on specific risk assessments.
How should carcinogenic chemical waste be labeled before disposal?
"Non-hazardous"
"Chemical Waste"
"General Trash"
"Carcinogen - Hazardous Waste"
Carcinogenic chemical waste must be clearly labeled as "Carcinogen - Hazardous Waste" to ensure special handling and disposal procedures. Generic labels do not convey the specific hazard. Proper labeling prevents accidental exposure.
Which bacterial pathogen is commonly screened in rabbits by serology to prevent respiratory disease?
Clostridium perfringens
Pasteurella multocida
Staphylococcus aureus
Escherichia coli
Pasteurella multocida is a primary pathogen in rabbits causing respiratory disease (snuffles). Facilities monitor serologically to detect and control outbreaks. Early detection reduces morbidity.
What behavioral sign can indicate distress in non-human primates?
Increased grooming of cage
Prolonged sleep
Self-directed behaviors like hair plucking
Increased eating
Self-directed behaviors such as hair plucking or self-biting are indicators of distress in non-human primates. Other signs include pacing and abnormal postures. Monitoring behavior is essential for welfare.
Which gas accumulation in rodent cages can indicate inadequate ventilation?
Nitrogen
Ammonia
Helium
Oxygen
Ammonia builds up from urine decomposition when ventilation is insufficient, causing respiratory irritation. Routine monitoring and cage changes help maintain air quality. Other gases listed are not typical by-products.
What is the minimum temperature and time cycle for sterilizing waste in an autoclave?
121°C for 5 minutes
100°C for 30 minutes
121°C for 15 minutes
134°C for 1 minute
A standard autoclave cycle of 121°C for at least 15 minutes at 15 psi is required to achieve sterilization of biohazardous waste. Shorter or lower temperature cycles do not ensure complete inactivation.
In health monitoring, what does ELISA stand for?
Enzyme-Linked Inhibitory Assay
Enzyme-Linked Immunosorbent Assay
Electrical Impedance Spectrometry
Environmental Linked Immunoassay
ELISA stands for Enzyme-Linked Immunosorbent Assay, a sensitive technique for detecting antibodies or antigens in biological samples. It is widely used in sentinel health monitoring for laboratory animals.
Under the Animal Welfare Act, which pain category requires the use of pain-relieving drugs unless justified otherwise?
Category B
Category A
Category C
Category D
Under USDA regulations, Category D includes experiments that may cause pain or distress, requiring use of analgesics and anesthetics unless scientifically justified. Category C involves procedures without pain but includes no anesthesia.
What is the first step in a facility leak investigation protocol?
Cleaning the area
Containing the leak
Writing a report
Notifying staff
The initial priority in a leak investigation is to contain the spill or leak to prevent spread of contaminants or hazards. Once contained, staff can proceed with cleaning, notification, and documentation.
When drafting a Standard Operating Procedure (SOP), which element is essential?
Font type
Purpose and scope
Paper size
Color scheme
An SOP must define its purpose and scope clearly to guide users on what the document covers and its intended outcomes. Procedural steps, responsibilities, and safety measures are built around this foundation.
How often should autoclave performance be validated with biological indicators?
Monthly
Daily
Biennially
Annually
Monthly biological indicator testing ensures that autoclaves effectively kill spores under actual load conditions. Daily chemical indicators confirm cycle parameters but do not prove sterilization. Regular validation maintains equipment reliability.
What is the primary action when a power failure occurs in an animal housing room?
Restart all experiments
Reset thermostats only
Evacuate animals
Check animal well-being and backup power
During a power failure, the first step is to assess animal welfare and verify activation of emergency backup power systems for ventilation and lighting. Experiments may resume once conditions are stable. Evacuation is rarely needed unless life support fails.
In semi-annual facility inspections, what is the ALAT's primary documentation duty?
Purchase supplies
Train staff
Design facility
Record observations and corrective actions
ALATs assist in semi-annual inspections by documenting facility condition, identifying deficiencies, and noting corrective actions. Accurate records support regulatory compliance and continuous improvement.
What distinguishes USDA pain Category B from Category C in animal use protocols?
Category B involves no pain or distress procedures, whereas Category C includes procedures without analgesia or anesthesia
Category B includes invasive surgery, Category C is observational only
Category B requires anesthesia, Category C does not
Category B applies to rodents, Category C to non-rodents
Under USDA regulations, Category B covers animals bred or held but not used in procedures that cause more than momentary pain or distress. Category C includes studies without anesthesia but with potential pain, requiring justification. This classification guides analgesic use.
Which BSL-2 practice is crucial when handling sharps contaminated with biohazardous material?
Hand recapping needles
Clean then reuse sharps
Drop sharps into regular trash
Use mechanical devices (forceps) to handle sharps when feasible
To reduce needlestick injuries, BSL-2 guidelines recommend using tools like forceps for sharps handling and avoiding manual recapping. Proper disposal in sharps containers is essential.
What enrichment strategy is recommended for group-housed pigs to promote natural behaviors?
Isolating animals
Providing rooting substrate like straw
Removing bedding
Using metal chains only
Pigs are natural foragers and rooting is an important behavior; providing straw or soil supports welfare and reduces stress-related behaviors. Metal chains alone are insufficient.
In water quality logs, a sudden spike in hardness may indicate what issue?
Low pH
Excessive chlorine use
High temperature
Filter failure
A sudden increase in water hardness often signals that ion-exchange filters or water softeners are malfunctioning. Hard water can affect animal health and equipment. Routine water quality monitoring helps detect such failures early.
When validating a new barrier room, which data should be reviewed before animal introduction?
Only temperature logs
Only light intensity measurements
Particle counts, airflow rates, pressure differentials
Only cleaning records
Validation of barrier rooms requires verification of particle counts, proper airflow rates, and consistent pressure differentials to ensure containment. Temperature and humidity are important but alone are not sufficient.
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Study Outcomes

  1. Understand Lab Animal Care Fundamentals -

    Grasp key principles of lab animal welfare and handling required for successful ALAT certification preparation.

  2. Apply Standard ALAT Protocols -

    Implement proper procedures for sanitation, restraint, and health monitoring in alignment with ALAT testing guidelines.

  3. Identify Common ALAT Test Scenarios -

    Recognize typical question formats and practical scenarios found in ALAT practice tests to improve recall and response accuracy.

  4. Analyze Performance Metrics -

    Examine your quiz results to pinpoint strength areas and knowledge gaps that need further review before the certification exam.

  5. Interpret Feedback for Improvement -

    Use detailed explanations and score tracking to refine your understanding of complex protocols and best practices.

  6. Evaluate Certification Readiness -

    Assess your overall preparedness and confidence level, ensuring you're ready to succeed on the official ALAT certification exam.

Cheat Sheet

  1. Safe Handling and Restraint -

    Mastering gentle restraint techniques - such as the scruff method for mice or the tunnel method - minimizes stress and prevents injury (NIH Guide, 2011). Practice controlled movements and proper hand positioning to ensure both your safety and the animal's welfare. Remember the "slow, steady, secure" mantra when approaching any small rodent.

  2. Aseptic Technique and Sanitation -

    Maintaining a sterile workspace and following a validated disinfection schedule (e.g., 10% bleach soak for 10 minutes) is crucial to prevent pathogen transmission (OLAW, 2020). Use the "clean-to-dirty" workflow: start with the cleanest cages and equipment before moving to higher-risk areas. Always don fresh gloves between handling different species or rooms.

  3. Accurate Animal Identification and Record-Keeping -

    Consistent, legible records are the backbone of any ALAT certification protocol (AALAS, 2022). Adopt the REDCAP mnemonic: Records, Environment, Diet, Count, Administration, and Parameters to ensure comprehensive data entry. Cross-check ear tags or microchip IDs at each shift change to avoid errors.

  4. Basic Anatomy and Physiology -

    Know key vitals: rodents' normal heart rate ranges from 300 - 700 bpm and respiratory rate 60 - 200 breaths per minute (Felasa Guidelines, 2019). Familiarize yourself with anatomical landmarks for injections - e.g., the quadriceps for intramuscular delivery in rats. Use flashcards or diagrams to reinforce organ systems and common clinical signs.

  5. Regulatory Compliance and Ethical Principles -

    Understand the 3Rs - Replacement, Reduction, Refinement - as outlined in the Guide for the Care and Use of Laboratory Animals (NRC, 2011). Stay current with USDA and PHS policy updates to ensure your ALAT certification practices meet federal standards. A quick "3R" checklist before procedures can boost your confidence and compliance.

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