PH quiz
Embryo Culture pH Knowledge Quiz
Test your understanding of pH regulation in embryo culture and sperm handling! This quiz will challenge your knowledge on buffer systems, sperm motility, and best practices in embryo management.
- 10 engaging multiple-choice questions
- Assess your knowledge in reproductive biology
- Learn about the crucial role of pH in embryo culture
What buffer system is used in embryo culture medium?
HEPES
Sodium bicarbonate
PBS
What buffer system is used to prepare washed sperm samples
HEPES
Sodium bicarbonate
MOPS
What is a zwitterion
A molecule with no charge
A molecule with a negative charge
A molecule with a positive charge
A molecule with both a positive and a negative charge
If your tube of culture medium, for the next day, is buffered with sodium bicarbonate, should the tube cap be loose or tight in a CO2 incubator?
Loose
Tight
There are 2 OPUs, it is 07:30 hr and the first OPU has not had dishes set up for. What do you do?
Cancel the OPU
Ask the team to delay the OPU until the dishes equilibrate
Quickly label a dishes and collect the oocytes in warmed HEPES buffered media from the incubator
Panic and call the lab director on their day off
Under CO2 conditions, take 50% of the equilibrated media from the dishes for the 2nd OPU and place into labeled dishes for the first patient
Does pH affect sperm motility
No
Yes
Sometimes
Sperm can tolerate:
A slightly more acidic pH
A slightly more alkaline pH
What is the best way to maintain gametes or embryos outside of the incubator?
With HEPES and then I can take as long as I want
Work quickly all of the time
Squeeze and hold the lid really tightly so no gas can escape
Your lab has been moved to Death Valley which is below sea level. You need to:
Increase the CO2 to the incubators
Decrease the CO2 to the incubators
At what stage is the oocyte/embryo most vulnerable to pH? (pick the BEST answer)
Blastocyst stage
4 -cell stage
Right after OPU
Between stripping and ICSI
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