Exam bio, part 2

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Biology Exam: Part 2

Test your knowledge in biology with our comprehensive quiz designed for students and enthusiasts alike. This quiz covers various topics, including cell biology, genetics, and molecular biology, ensuring a holistic understanding of the subject.

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23 engaging questions
Multiple choice and checkbox formats
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23 Questions6 MinutesCreated by ExploringCells42

Which one of the following organelles digests the proteins that are no longer useful to the cells?

Lysosomes

Mitochondria

Nucleus

Plasma membrane

The exchange of segments of non-sister chromatids between chromosomes of a homologous pair is termed:

Translocation

Transformation

Crossing-over

Chromosomal aberration

What is true?

cDNA contains exons and introns of the genes.

DNA sequence that code for protein are known as exons

The organelles in the cell where protein synthesis occurs are mitochondria

DNA is made of two chains that twist about one another in the shape of double helix

Which enzymes are crucial for inhibition of protein synthesis?

DNA polymerase

DICER

RNA polymerase

Ligase

Mitochondria are;

Involved in the secretion of proteins

Membrane bound structures specialized for digestion

Energy processing organelles

Storing fat

If an organism has 46 chromosomes in its somatic cells it comes from a germline cell that contains chromosomes:

What is true:

Nucleotides are the building blocks of proteins

DdNTP are used in the sequencing

The S-phase of a cell-cycle is directly after the G2-phase

�The sequence element on the DNA template which is first recognized by RNA polymerase is known promoter region.

Amino acids are the building blocks of proteins

The S-phase of a cell-cycle is directly after the G1-phase

A promoter on the DNA is recognized by ... 1........... to .........2.........

1: DNA polymerase, 2: stop transcription

1: RNA polymerase, 2: start transcription

1: RNA polymerase, 2: stop transcription

1: DNA polymerase, 2: start transcription

What is true?

The transfer of information from DNA to RNA is referred to as translation

A codon is a group of 4 nucleotides that codes for ne amino acid

Translation occurs in eukaryotes on ribosomes located in the Golgi body

Silent mutations have no effect on the phenotype

Denaturation involves:

Heating between 90 and 98 degrees Celcius

Heating between 40 and 60 degrees Celcius

Heating between 70 and 72 degrees Celcius

Helicase enzyme

Maldi-Toff mass array (Sequenom):

Is performed on the basis of genomic DNA

Is performed on the basis of PCR prodcuts

Requires electrophoresis

Can detect DNA polymorphism

During PCR:

72 degrees Celsius is required for DNA denaturation

94 degrees Celsius is required for DNA denaturation

94 degrees Celsius is required for primer annealing

72 degrees Celsius is required for Taq polymerase elongation

48-72 degrees Celsius is required for primer annealing

It is true that:

Crossing-over leads o balanced translocation

Karyotyping can be performed on the basis of paraffin-embedded tissues

Detergents are need for RNA isolation

DNA polymerase is used during DNA isolation

RT-PCR /Reverse-Transcription PCR):

Requires restriction enzymes

Is not only non-specific but also time- consuming procedure

Is performed on the basis of genomic DNA

Requires reverse transcriptase enzyme

Mark techniques used in detection of 10000 bp nucleotide deletion

Real-Time PCR with simple-probes

Sequencing

Southern-blot

Real-Time PCR with Taq-Man probes

Biopsy of breast tumor revealed breast cancer.

Chest computer tomography has to be performed in search of potential

Metastases- Next visit in half a year is recommended

Surgery has to be performed as soon as possible

Surgery can be performed within a period of six months

Gene therapy:

Is frequently used in treatment of metastatic melanoma patients

Can be based upon antisense strategy

Is highly effective in cancer treatment

Is not a routine way of cancer treatment

Klinefelter syndrome:

Is caused by mutation

Is caused by unbalanced translocation

Can be diagnosed by karyotyping

Can be diagnosed by sequencing

Ligase could be expected to do which of the following?

Join DNA fragments by catalyzing the formation of a phosphodiester bond

Reform broken hydrogen bonds

Break phosphodiester bonds

Add nucleotides to an existing RNA molecule

During the extension step of PCR, which enzyme binds to the PCR primer and adds nucleotides?

DNA Elongase

RNA primase

Taq polymerase

Helicase

Which out of the following is an example of post translation modification?

Subunit aggregation

Splicing

Base modification

Mutation

You have in your possession a microscope slide with meiotic cells on it and a light microscope. What would you look for if you wanted to identify metaphase 1 (meiosis) cells on the slide?

A visible nuclear envelope

Separated sister chromatids at each pole of the cell

Tetrads lined up at the center of the cell

Euchromatin

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Exam bio, part 2

Biology Exam: Part 2

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