Exam bio, part 2
Biology Exam: Part 2
Test your knowledge in biology with our comprehensive quiz designed for students and enthusiasts alike. This quiz covers various topics, including cell biology, genetics, and molecular biology, ensuring a holistic understanding of the subject.
Features:
- 23 engaging questions
- Multiple choice and checkbox formats
- Instant feedback on your answers
Which one of the following organelles digests the proteins that are no longer useful to the cells?
Lysosomes
Mitochondria
Nucleus
Plasma membrane
The exchange of segments of non-sister chromatids between chromosomes of a homologous pair is termed:
Translocation
Transformation
Crossing-over
Chromosomal aberration
What is true?
cDNA contains exons and introns of the genes.
DNA sequence that code for protein are known as exons
The organelles in the cell where protein synthesis occurs are mitochondria
DNA is made of two chains that twist about one another in the shape of double helix
Which enzymes are crucial for inhibition of protein synthesis?
DNA polymerase
DICER
RNA polymerase
Ligase
Mitochondria are;
Involved in the secretion of proteins
Membrane bound structures specialized for digestion
Energy processing organelles
Storing fat
If an organism has 46 chromosomes in its somatic cells it comes from a germline cell that contains chromosomes:
6
12
23
46
What is true:
Nucleotides are the building blocks of proteins
DdNTP are used in the sequencing
The S-phase of a cell-cycle is directly after the G2-phase
€™The sequence element on the DNA template which is first recognized by RNA polymerase is known promoter region.
Amino acids are the building blocks of proteins
The S-phase of a cell-cycle is directly after the G1-phase
A promoter on the DNA is recognized by ... 1........... to .........2.........
1: DNA polymerase, 2: stop transcription
1: RNA polymerase, 2: start transcription
1: RNA polymerase, 2: stop transcription
1: DNA polymerase, 2: start transcription
What is true?
The transfer of information from DNA to RNA is referred to as translation
A codon is a group of 4 nucleotides that codes for ne amino acid
Translation occurs in eukaryotes on ribosomes located in the Golgi body
Silent mutations have no effect on the phenotype
Mature mRNA contains:
Exons
Anticodons
Promoter
Introns
Denaturation involves:
Heating between 90 and 98 degrees Celcius
Heating between 40 and 60 degrees Celcius
Heating between 70 and 72 degrees Celcius
Helicase enzyme
Maldi-Toff mass array (Sequenom):
Is performed on the basis of genomic DNA
Is performed on the basis of PCR prodcuts
Requires electrophoresis
Can detect DNA polymorphism
During PCR:
72 degrees Celsius is required for DNA denaturation
94 degrees Celsius is required for DNA denaturation
94 degrees Celsius is required for primer annealing
72 degrees Celsius is required for Taq polymerase elongation
48-72 degrees Celsius is required for primer annealing
It is true that:
Crossing-over leads o balanced translocation
Karyotyping can be performed on the basis of paraffin-embedded tissues
Detergents are need for RNA isolation
DNA polymerase is used during DNA isolation
RT-PCR /Reverse-Transcription PCR):
Requires restriction enzymes
Is not only non-specific but also time- consuming procedure
Is performed on the basis of genomic DNA
Requires reverse transcriptase enzyme
Mark techniques used in detection of 10000 bp nucleotide deletion
Real-Time PCR with simple-probes
Sequencing
Southern-blot
Real-Time PCR with Taq-Man probes
Biopsy of breast tumor revealed breast cancer.
Chest computer tomography has to be performed in search of potential
Metastases- Next visit in half a year is recommended
Surgery has to be performed as soon as possible
Surgery can be performed within a period of six months
Gene therapy:
Is frequently used in treatment of metastatic melanoma patients
Can be based upon antisense strategy
Is highly effective in cancer treatment
Is not a routine way of cancer treatment
Klinefelter syndrome:
Is caused by mutation
Is caused by unbalanced translocation
Can be diagnosed by karyotyping
Can be diagnosed by sequencing
Ligase could be expected to do which of the following?
Join DNA fragments by catalyzing the formation of a phosphodiester bond
Reform broken hydrogen bonds
Break phosphodiester bonds
Add nucleotides to an existing RNA molecule
During the extension step of PCR, which enzyme binds to the PCR primer and adds nucleotides?
DNA Elongase
RNA primase
Taq polymerase
Helicase
Which out of the following is an example of post translation modification?
Subunit aggregation
Splicing
Base modification
Mutation
You have in your possession a microscope slide with meiotic cells on it and a light microscope. What would you look for if you wanted to identify metaphase 1 (meiosis) cells on the slide?
A visible nuclear envelope
Separated sister chromatids at each pole of the cell
Tetrads lined up at the center of the cell
Euchromatin
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