Lab animal Final
Lab Animal Safety and Care Quiz
Test your knowledge on laboratory animal safety, care, and handling with this comprehensive quiz! Designed for anyone involved in animal research, this quiz covers a wide range of topics including biosafety levels, nutrition, and hygiene practices.
Key features:
- 99 engaging questions
- Multiple choice format
- Designed for both beginners and experts
A standard HEPA filter is 99.97% efficient in removing particles of 0.3 μm diameter. What is the efficiency of a standard HEPA filter in removing particles less than 0.3 μm in diameter?
99.97% efficiency
less than 99.97% efficiency
greater than 99.97% efficiency
True or False: Laminar change stations DO NOT provide personnel a greater degree of protection against exposure to airborne particles than is provided by a biological safety cabinet.
True
False
Animal biosafety level designation that involves the highest level of risk is
ABSL1
ABSL2
ABSL3
ABSL4
_________ provide(s) the most effective and practical measures to reduce personnel's exposure to laboratory animal allergens.
Personal protective equipment
Engineering controls
Operational procedures and practices
Recommended relative humidity range for mouse housing rooms is
20%–50%.
30%–70%.
40%–80%.
10%–40%.
For which of the following rooms should airflow be directed into the room from an adjacent hallway?
surgery room
animal housing room with ABSL2 containment
personnel break room
clean cagewash area
Interstitial areas allow building support personnel to access select building components without entering the animal facility.
are ideal locations for housing large animals.
are recommended only for single”story facilities.
allow building support personnel to access select building components without entering the animal facility.
are necessary to support directional airflow within a facility.
The guideline for ventilation in animal housing rooms is ___________ air changes per hour
8-10
10-15
4-8
15-20
A _______ should be conducted to estimate the potential health risk of all who may enter an animal facility or who may have contact with animals and equipment.
risk assessment
job analysis
physical exam
health needs assessment
ATP bioluminescent technology is used
to assess the efficacy of sanitation procedures.
to balance room air pressure.
to calculate room humidity levels.
to determine facility airflow.
Potential risks to the health of cagewash personnel include
performance of repetitive motions and heavy equipment movement.
exposure to laboratory animal allergens.
noise, heat and chemical exposure.
All of the above
Most dry animal diets that are properly stored can be used up to _____ after milling.
9 months
6 months
3 months
1 year
Personnel protection and environmental containment of biohazardous materials can be achieved through use of a three”tiered system utilizing personal protective equipment (PPE), operational procedures and practices, and building and engineering controls. In what order should these systems be employed?
PPE first, followed by building and engineering controls, and finally operational procedures and practices
building and engineering controls first, followed by operational procedures and practices, and finally PPE
operational procedures and practices, followed by building and engineering controls, and finally PPE
PPE first, followed by operational procedures and practices, and finally building and engineering controls
A squeeze cage is routinely used for these animals.
ferrets
Rabbits
Nonhuman primates
Chinchillas
The cage type used to separate and collect urine and feces is
Squeeze
Gang
Metabolism
microisolator
Disinfection
is the maintenance of environmental conditions conducive to health and well”being.
is a validated process to render a product free of all forms of viable microorganisms.
only removes excessive amounts of excrement, dirt, and debris.
reduces or eliminates unacceptable concentrations of microorganisms.
Type of cage washing equipment designed to sanitize large pieces of equipment such as a cart is
Cabinet washer
Rack washer
Dish washer
Tunnel washer
Temperature suggested to achieve sanitation in an automated cage washer is
180°F
280°F.
250°F.
160°F.
The cost to maintain one animal (or one cage of animals) for one day is called
per annum.
perdu.
per diem.
per capita.
The _______ requires employers to provide safe and healthy working conditions for their employees.
Occupational Safety and Health Standards
Occupational Protection Standards
Safe Employee Health Standards
Fair Employment Health Standards
True or False: Laminar change stations provide personnel a greater degree of protection against exposure to airborne particles than is provided by a biological safety cabinet.
True
False
Which is NOT an acceptable reason to singly house animals of a social species?
veterinary concerns regarding animal well”being
observed social incompatibility (e.g., fighting)
scientific necessity
Cost
Recommended methods for the individual identification of adult rats include all but which of the following:
toe clipping
microchip implantation
tattooing
ear tagging
As a general guideline, up to ______% of the circulating blood volume can be withdrawn every 2–3 weeks from normal, healthy animals with minimal effect.
7
5
10
12
Which of the following is INCORRECT regarding rat nutrition?
Rats tend to become obese if fed ad libitum throughout adulthood.
Rats eat an average of 5–6 g of rodent chow per 100 g of body weight per day.
Rats drink an average of 10–12 mL of water per day.
Rats are copraphagic.
What is the usual gestation period of a rat?
21–23 days
19–21 days
23–25 days
17–19 days
What is the name of the AVMA guidance document for members of the veterinary profession who carry out or oversee the euthanasia of animals?
Guidelines for Laboratory Animal Euthanasia for Research
Guidelines for the Euthanasia of Animals
Guidelines for the Practice of Euthanasia
Guidelines for the Euthanasia of Research Animals
If a procedure is known to cause pain in humans, it must be assumed to also cause pain in rats unless ___________.
it is clinically insignificant
it negatively affects research outcomes
it is of short duration
proven otherwise
Which genetic classification is useful for modeling natural population diversity?
outbred stock
inbred strain
genetically engineered
_______ animals colonized only with a known population of microflora.
Axenic
Germ”free
Specific pathogen–free
Gnotobiotic
Recommended site for administration of intraperitoneal injections in rats is
upper right abdominal quadrant.
lower right abdominal quadrant.
upper left abdominal quadrant.
lower left abdominal quadrant.
Which type of nesting material should be avoided in nude mice?
soft paper towels
paper twists
facial tissues
cotton
Mice are social animals and do best when housed in small, compatible groups
True
False
It is unnecessary for mice to be anesthetized for retro”orbital blood collection or injections.
True
False
Ear Punching is an invasive tissue collection technique for genotyping that does requires anesthesia or analgesia.
True
False
What percentage of body weight is the total blood volume in mice and rats?
0.7%
10%
7%
5%
Mats and rice are born "altrical"?
True
False
Mice are social animals and do best when housed in small, compatible groups
True
False
Recommended site for administration of intraperitoneal injections in rats is upper right abdominal quadrant.
True
False
What organs could be damaged by improper intraperitoneal [IP] injection technique in the rat?
kidney
Heart
Stomach
liver, bladder, or cecum
It is not acceptable to scruff a rabbit on the back of the neck behind the ears.
True
False
Ferrets are too agile for scruffing to be an effective part of restraint.
True
False
Injury to a ferret's back is not a concern during restraint because their spine is so flexible.
True
False
Skunks infected with rabies may not show any symptoms for up to:
10 days
2 weeks
2 months
6 months
If you are holding an animal such as a racoon by the scruff, they will not be able to turn and bite you.
True
False
Like many other rodents, prairie dogs can be scruffed by the extra skin on the back of the neck.
True
False
The spikes on an obese hedgehog are pushed close together, so you will not see any skin between them.
True
False
Capture myopathy can cause permanent muscle damage and death.
True
False
The method for holding a guinea pig in your arms is very much like holding a rabbit.
True
False
Which is NOT an acceptable way to handle a rabbit?
With the rabbit on the table, use your forearm to gently tuck their body against you.
Support the rabbit's hind end and feet when holding them.
Gently "scruff" the skin on the back of the neck behind the ears.
Pick up the rabbit by the ears, then support their hind feet to prevent kicking.
Which statement is NOT true regarding the fur of chinchillas?
Large bald patches can result if handled too roughly.
Mold can grow in the fur if not dried thoroughly.
Lava dust is important to the health of the fur.
Salt baths are required to keep the fur clean.
What is a chinchilla's defense mechanism that causes them to lose large patches of fur?
Loose fur
Balding defense
Fur slip
Dander mechanism
What is the main reason you should never pick up a rodent by the end of their tail?
They can turn and bite you.
They will scratch you with their hind feet.
The skin will come off of the tail.
The tail is too slippery.
When making general observations while handling a calm, friendly ferret:
You should begin by scruffing them along the back of the neck.
Start by wrapping them in a towel.
Hold them under the chest and allow the lower half of the body to hang down, so the back is straight.
While supporting them under the chest, either tuck their lower body into yours or use your other hand to support the hips.
Wild animals often hide signs of illness until the disease is serious.
True
False
Chinchillas can become overheated if handled for long periods.
True
False
All of these methods are helpful in getting a good look at the underside of a hedgehog EXCEPT:
Allowing the hedgehog to walk across clear plexiglass.
Administering sedation or anesthesia.
Manually open them from their ball using gloves.
Observe the hedgehog while the owner is handling them.
Rabbits have such a strong kick that they can break their own back.
True
False
Some rodents can be distracted with food while a procedure is performed.
True
False
Like guinea pigs, rabbits will often vocalize and "complain" when they are handled.
True
False
Name the the intestinal coccidia of guinea pigs,
Paraspidodera uncinata
Eimeria caviae
Trixacarus caviae
Trichophyton mentagrophytes
Tooth overgrowth in guinea pigs usually involves premolars and anterior molars.
True
False
The most common etiologic agent of ringworm in the guinea pig is:
Chirodiscoides caviae
Chlamydophila caviae
Trichophyton mentagrophytes
At the beginning of an anesthetic procedure, it necessary to rinse the oral cavity of guinea pigs because they frequently retain a considerable amount of feed in their buccal cavities, which can contribute to airway obstruction when they are anesthetized.
True
False
The gestation period of guinea pigs ranges from
71-80 days
29-34 days
59 to 72 days
45-55 days
Guinea pigs are do best when housed individually.
True
False
Subcutaneous (SC) injections require some force because guinea pig skin is tough, especially over the neck and back.
True
False
Guinea pigs have readily accessible peripheral veins ideal for blood collection.
True
False
Which antimicrobial is the LEAST toxic to administer to guinea pigs.
Cephalosporins
Fluoroquinolones
clindamycin
Penicillins
Which pathogen is associated with enteritis in 4–8”week”old kits?
Clostridium tetani
Clostridium perfringens
Clostridium spiroforme
Clostridium difficile
Which of the following is a zoonotic agent?
Fransicella tularensis
Clostridium spiroforme
Treponema paraluiscuniculi
Pasteurella multocida
The marginal ear vein is a small blood vessel that is easily accessible and useful for both blood withdrawal and intravenous injections.
True
False
Ringworm is commonly encountered in laboratory rabbits.
True
False
Which of the following is not due to a genetic mutation?
Mandibular prognathism
Sore hock
Buphthalmia
Splay leg
Which protozoa causes hepatic coccidiosis?
Eimeria perforans
Eimeria media
Eimeria stediae
Eimeria magna
Rabbit kits only need to nurse one time a day.
True
False
Which of the following is a reportable disease in the United States?
Tyzzer's disease
Encephalitozoonosis
Rabbit papilloma virus
Rabbit hemorrhagic disease
What is the most common neoplasm of rabbits?
Bile duct adenoma
Mammary adenocarcinoma
Lymphosarcoma
Uterine adenocarcinoma
The lumbar muscle site is preferred for intramuscular injection for rabbits.
True
False
Preanesthetic fasting is not required in this species due to their high metabolic rate.
True
False
Gerbil neonates are born precocious.
True
False
Just like hamsters, guinea pigs, and rabbits, many antibiotics are unsafe to use in gerbils due to the sensitivity of the intestinal microflora.
True
False
Why is it particularly important to control excess noise in gerbil housing areas?
It can induce cardiac arrest.
They are noise sensitive.
They can have epileptiform seizures.
It disturbs their mating ritual
What can result if a gerbil is restrained by the tail improperly?
The gerbil will bite you.
The gerbil will emit a loud squeal.
Nothing will happen.
The tail will deglove.
Since gerbils urinate a very small amount, often their cages need less frequent changing than other rodents.
True
False
What is the gestation length of a nonlactating gerbil?
20-23 days
32-35 days
24-26 days
28-31 days
What is the most ideal time to pair hamsters for successful breeding?
In the morning of the third day after the observance of the postovulatory discharge.
In the evening of the third day after the observance of the postovulatory discharge
The second day in the evening after the observance of the postovulatory discharge
It doesn't matter.
Per the Animal Welfare Act, it is acceptable to feed hamsters on the floor of the cage.
True
False
Hamsters are social species and should be group housed.
True
False
Which of the following is NOT a condition that is needed for a hamster to hibernate.
quietness
low light intensity
short day lengths
warmer temperatures
The most ideal time to pair hamsters for successful breeding is in the evening of the third day after the observance of the postovulatory discharge
True
False
Small”particle bedding materials are contraindicated in recovery cages because they can stick to the eyes, nose, and mouth of the recovering hamster and may be aspirated.
True
False
The following are all viral diseases that affect hamsters except:
Helicobacter
Hamster Polyomavirus
Lymphocytic Choriomeningitis Virus
Hamster Parvovirus
All of the following are methods to permanently identify individual gerbils except:
ear tagging
microchip
fur clipping
ear punching,
Rodents are not more prone to hypothermia than larger animals?
True
False
Guinea pigs lack the enzyme L-gulonolactone oxidase.
True
False
Which of the following rodents has hypsodontic teeth?
Hamster
Rabbit
Guinea Pig
Gerbil
Which mite most commonly affects the fur of rabbits?
Sarcoptes
Cheyletiella
Microsporum
Demodex
Which animal requires vitamin C in its diet?
Hedgehog
Rabbit
Sigar glider
Guinea Pig
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