DdPCR and QX One Quiz
ddPCR and QX One Quiz
Test your knowledge on digital droplet PCR (ddPCR) and the QX One system with this engaging quiz designed for professionals and enthusiasts alike.
Whether you are a researcher or a laboratory technician, this quiz will help reinforce your understanding of key concepts:
- ddPCR methodology
- Quantification techniques
- QX One cartridge features
DdPCR calculates the target concentration using what method?
Standard Curve
Poisson Distribution
Colony Counts
Predictive Analysis
What time of quantification does ddPCR provide?
Qualitative/Semi-Quantitative
Relative Quantitative
Absolute Quantification
What are the 4 channels that can be read on the QX One?
FAM/HEX/ROX/CY3
FAM/HEX/Atto425/CY5
FAM/HEX/CY5/CY5.5
FAM/HEX/TEXASRED/SybrGreen
What is the minimum number of droplets that are required for a well to be considered valid?
1,000
5,000
10,000
20,000
At what stage do the droplets become stable?
Droplet Generation
Droplet Reading
Thermocycling
What volume should each well of the GCR96 cartridge be filled with?
5uL
50uL
20uL
15uL
The GCR96 Cartridge is sealed once in the PX1 Plate Sealer?
True
False
Which setting cannot be changed after the plate has run?
Supermix
Plate Template
Plate name
Experiment Type
Thresholds for positive and negative droplets are typically set using which samples?
Negative Controls
Positive Controls
Test Samples
When setting the Threshold, which tool is used for applying the Threshold to the rest of the plate/assay?
Threshold Single Well
SD Multiple Wells
Threshold Multiple Wells
Auto Threshold
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