<div><strong>SEQUENCE A DNA GAME STRUCTURE</strong></div>

Create an educational illustration depicting various aspects of molecular biology and DNA sequencing techniques, featuring DNA strands, lab equipment, and colorful visual representations of molecular processes.

Molecular Biology Mastery Quiz

Test your knowledge in the fascinating field of molecular biology with our comprehensive quiz! Covering essential topics such as DNA structure, sequencing techniques, and applications, this quiz is designed for learners of all levels.

Challenge yourself with questions that explore:

The Central Dogma of Molecular Biology
Next Generation Sequencing (NGS) workflows
Applications in genomics

10 Questions2 MinutesCreated by AnalyzingCell417

Presentation 1: Introduction to Molecular Biology and Techniques

Among the four basic biomolecules, which one has amino acids as its monomeric units?

Proteins

Carbohydrates

Nucleic acids

Lipids

The Central Dogma of Molecular Biology describes how information is stored and transferred inside the cell. Which of the following below is the correct sequence of the central dogma?

Translation > Transcription > Replication

Translation > Replication > Transcription

Transcription > Replication > Translation

Replication > Transcription > Translation

Which DNA extraction method uses phenol-chloroform-isoamyl alcohol as solvent and is governed by the Nernst Law of Distribution?

Organic Solvent

Spin Column

Magnetic Bead

Fluorometry

Electrophoresis is a DNA separation technique that uses an electric field. To make this possible, it uses a compound that serves as a molecular “sieve” of “filter” in electrophoresis. What do you call this specific compound?

Agarose Gel

Fluorophore

Silica

Elute

Presentation 2: Introduction to NGS and Illumina Workflow

In the general workflow of Next Generation Sequencing, library preparation is the first step. To produce a functional library, the following are the key procedures, except:

Shearing or Fragmentation

Adapter Ligation

Template Preparation

Size selection

In sequencing strategies, this refers to the number of times a single base position was sequenced.

Multiplexing

Coverage

Fragmentation

Ligation

Ligation technology is used to construct NGS libraries for sequencing. This process uses an enzyme to connect specialized adapters to both ends of the DNA fragments; thereby, allowing the following except:

Substrate fusion

Amplification

Attachment to substrate

Attachment of sequencing primers

Presentation 3: Applications of NGS

Major developments in the sequencing technology constituted the rise of Second-Generation Sequencers which makes use of massively parallel sequencing methods. Which of the following does not belong to second generation sequencing?

Pyrosequencing

Ion Torrent

Oxford Nanopore

Illumina

TRUE or FALSE: There are two major applications of next generation sequencing (NGS) in Genomics, which are (1) Whole Genome Sequencing (WGS) and (2) Targeted Sequencing. In the discussion in Lesson 3: Applications of NGS, common library preparation strategies were also discussed with Shotgun and Mate Pair as an example on WGS.

TRUE

FALSE

In COVID-19 management, the Philippine Genome Center (PGC) utilizes Illumina COVIDSeq Assay as a diagnostic test for COVID-19. Which of the following correctly showcases the sequence of laboratory processing of SARS-CoV-2 using Illumina COVIDSeq Test?

Library Preparation > RNA Extraction > PCR Enrichment > Sequencing

RNA Extraction > PCR Enrichment > Library Preparations > Sequencing

PCR Enrichment > RNA Extraction > Library Preparations > Sequencing

PCR Enrichment > Library Preparation > RNA Extraction > Sequencing

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SEQUENCE A DNA GAME STRUCTURE

Molecular Biology Mastery Quiz

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