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Quizzes > Science

9th Grade Biology Practice Test for Honors Students

Quick, free honors biology practice test. Instant feedback.

Editorial: Review CompletedCreated By: Chanelle HusbandsUpdated Aug 26, 2025
Difficulty: Moderate
2-5mins
Learning OutcomesCheat Sheet
Paper art illustration for 9th Grade Honors Biology practice test on teal background

This 9th grade honors biology quiz helps you review cells, genetics, and ecology and see where to focus before the exam. For broader practice, try our high school biology quiz or step up to a grade 10 biology quiz; advanced learners can sample an ap biology unit 2 quiz.

What is the primary function of mitochondria in eukaryotic cells?
Photosynthetic sugar production
Lipid packaging and export
Synthesis of polypeptides
ATP production through aerobic respiration
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Which component forms the fundamental structure of the cell membrane?
Peptidoglycan layer
Phospholipid bilayer
Cellulose microfibrils
Chitin sheets
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What type of bond holds complementary bases together in a DNA double helix?
Covalent bonds between bases
Peptide bonds
Hydrogen bonds
Ionic bonds
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Which macromolecule is the primary long-term energy storage in animals?
Lipids
Nucleic acids
Starches only
Proteins
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Which organelle modifies, sorts, and packages proteins for secretion or delivery?
Centrosome
Nucleolus
Golgi apparatus
Smooth endoplasmic reticulum
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The movement of water across a semipermeable membrane from high water potential to low water potential is called
Exocytosis
Endocytosis
Active transport
Osmosis
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Ribosome subunits are assembled in which nuclear structure?
Nuclear pore
Nuclear lamina
Chromatin
Nucleolus
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Which stage of cellular respiration produces the most ATP per glucose molecule?
Electron transport chain and chemiosmosis
Glycolysis
Citric acid cycle alone
Link reaction (pyruvate oxidation)
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Which statement best describes facilitated diffusion across membranes?
Solutes move down their concentration gradient via specific transport proteins without energy input
Water moves across membranes via aquaporins using ATP
Solutes move against their gradient using ATP and carrier proteins
Large particles are engulfed into vesicles by membrane infolding
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A dihybrid cross between two individuals heterozygous for both traits (AaBb x AaBb) typically yields which phenotypic ratio?
1:1
3:1
9:3:3:1
1:2:1
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If a DNA template strand reads TAC, what codon will appear on the mRNA?
GUA
UAC
AUG
ATG
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In energy pyramids, approximately what fraction of energy is transferred from one trophic level to the next?
About 50%
About 90%
About 10%
About 1%
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Which biogeochemical cycle generally lacks a significant gaseous phase under natural conditions?
Water cycle
Carbon cycle
Nitrogen cycle
Phosphorus cycle
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Population growth that slows as it approaches carrying capacity is best described as
Arithmetic growth
Exponential growth
Boom-and-bust growth
Logistic growth
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What term describes the loss of a protein's native shape due to heat or pH extremes, resulting in loss of function?
Condensation
Phosphorylation
Denaturation
Hydrolysis
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Which meiotic event directly increases genetic variation by exchanging DNA between homologs?
Crossing over during prophase I
Spindle formation during metaphase II
Nuclear envelope breakdown in prophase II
Cytokinesis after telophase I
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Which pigment in photosystem II primarily absorbs light to initiate photosynthesis?
Chlorophyll b
Carotene
Chlorophyll a
Xanthophyll
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Which cytoskeletal structures form the spindle fibers that separate chromosomes during mitosis?
Microfilaments
Actin-myosin bundles
Intermediate filaments
Microtubules
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Which pH value represents a weak acid?
pH 2
pH 5
pH 8
pH 7
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Noncoding RNA segments removed from a pre-mRNA transcript before translation are called
Introns
Codons
Promoters
Exons
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Study Outcomes

  1. Understand Cell Structure and Function -

    Explain the roles of major cell organelles, compare prokaryotic and eukaryotic cells, and apply this knowledge to challenging cell biology questions.

  2. Apply Genetic Principles -

    Solve inheritance problems using Punnett squares, predict phenotypic outcomes, and interpret patterns of Mendelian and non-Mendelian genetics.

  3. Analyze Ecological Interactions -

    Interpret food webs and energy flow diagrams, describe biotic and abiotic factors, and assess population dynamics within ecosystems.

  4. Evaluate Experimental Data -

    Critically assess graphs, tables, and experimental designs, draw valid biological conclusions, and identify sources of error in lab scenarios.

  5. Identify Knowledge Gaps -

    Use instant feedback to pinpoint areas for review, prioritize study topics, and track improvement across cell biology, genetics, and ecology.

  6. Build Test-Taking Strategies -

    Develop effective techniques for the honors biology midterm, final, and EOC exams, manage time under pressure, and increase overall confidence.

Cheat Sheet

  1. Organelle Functions and the Fluid Mosaic Model -

    Cells contain organelles like mitochondria for ATP production and the Golgi apparatus for protein packaging. According to Campbell Biology (Pearson), the fluid mosaic model describes a phospholipid bilayer with embedded proteins that regulate transport. Use the mnemonic "Mighty Mighty Mitochondria" to remember ATP factories.

  2. Membrane Transport and Tonicity -

    Passive transport like diffusion and osmosis moves substances down concentration gradients without energy, while active transport uses ATP to move molecules against gradients (Campbell Biology). Remember "P for Passive - Passenger ride, no extra energy; A for Active - Active uses ATP." This concept is fundamental for honors biology 9th grade practice test questions on cell homeostasis.

  3. Mendelian Genetics and Punnett Squares -

    Mendel's laws of segregation and independent assortment predict genotype ratios using monohybrid (3:1) and dihybrid (9:3:3:1) Punnett squares (NCBI). Use FOIL (First, Outside, Inside, Last) to organize dihybrid crosses easily. Mastering these basic ratios will boost your score on any biology honors eoc practice test.

  4. Photosynthesis and Cellular Respiration Equations -

    Photosynthesis converts light energy in chloroplasts: 6CO2 + 6H2O → C6H12O6 + 6O2, while cellular respiration in mitochondria reverses this to produce ~36 ATP per glucose (Nature Education). Mnemonic "OIL RIG" (Oxidation Is Loss, Reduction Is Gain) helps track electron flow in redox reactions. These equations are staples on your honors biology midterm practice test.

  5. Energy Flow and Trophic Levels -

    Energy moves through ecosystems via producers, consumers, and decomposers, with only ~10% transfer efficiency at each trophic level (U.S. Forest Service). Visualize a pyramid and remember the "10% rule" to calculate available energy up the chain. This principle is key for questions on your honors biology final exam and ecology sections.

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