Week 2 revision quiz
Week 2 Revision Quiz: Mastering KARI-6xHis
Test your knowledge on the processes and key concepts related to the purification and analysis of KARI-6xHis. This quiz is designed to reinforce your understanding from Week 2 of our study program. Whether you're prepping for an exam or just looking to refresh your knowledge, this interactive quiz will help solidify your grasp of the material.
- 10 challenging questions
- Multiple-choice format
- Enhance your understanding of protein purification
Why do we add Lysozyme?
To digest proteins from the lysate
To break down the cell wall
To digest DNA
To make the cells grow
What does the induced cell lysate contain
KARI only
All proteins produced by the cell
KARI-6xHis only
6xHis only
What is the function of Binding Buffer
To bind the KARI-6xHis to the resin
To clean up the column
To cleave KARI-6xHis
To wash proteins that aren't bound to the resin
What is the function of wash buffer
To bind the KARI to the resin
To clean up the column
To cleave KARI-6His
To wash proteins that aren't bound to the resin
Why does the elution buffer contain the highest concentration of immidazole?
Immidazole binds to KARI-6xHis and precipitates it
Immidazole competitively binds to the resin thereby eluting KARI-6xHis
Immidazol cleaves the bond between KARI-6xHis and elutes only KARI
Immidazol denatures the His tag leading to elution
Immidazole unfolds KARI-6xHis leading to its elution
Why do we do a desalting step of the eluted fractions containing KARI-6xHis?
To get rid of random proteins
To get rid of KARI co factors
To get rid of the His tag
To get rid of any salts from the eluted fractions.
How does the Desalting column (G-25 Sephadex column work)?
Size exclusion (Smallest molecule out first)
Size exclusion (Biggest molecule out first)
Charge based elution (Highest charge out first)
Charge based elution (Lowest charge out first)
What does the BCA assay identify?
Only KARI proteins
Only His tagged proteins
All proteins in the solution
Only presence of salt contamination
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