Lecture 4 Quiz
Gene Cloning and Molecular Techniques Quiz
Test your knowledge on gene cloning, molecular biology techniques, and the role of plasmids in DNA manipulation with this engaging quiz! Whether you are a student or a researcher, this quiz will help solidify your understanding of important concepts in the field.
Topics covered include:
- Gene isolation methods
- Cloning processes
- Restriction enzymes
- Electrophoresis principles
By which of these method we can Isolate a gene of interest from total DNA?
DNA sequence analysis
UV Absorbance Spectrophotometry
Polymerase Chain Reaction (PCR)
Agarose Gel Electrophoresis
Which sequence is correct in the gene cloning process?
1) Prepare pure insert DNA (target or foreign) samples from donor cells or tissues and analyse. 2) Prepare vector DNA in which the target DNA will be inserted 3) Ligation (joining) of insert and vector DNA molecules together 4) Introduction of insert-vector recombinant construct into suitable host cells 5) Restriction enzyme digestion of target DNA and vector DNA 6) Growth, selection and identification of bacterial host cells containing recombinant DNA
1) Prepare vector DNA in which the target DNA will be inserted 2) Prepare pure insert DNA (target or foreign) samples from donor cells or tissues and analyse. 3) Ligation (joining) of insert and vector DNA molecules together 4) Restriction enzyme digestion of target DNA and vector DNA 5) Introduction of insert-vector recombinant construct into suitable host cells 6) Growth, selection and identification of bacterial host cells containing recombinant DNA
1) Prepare pure insert DNA (target or foreign) samples from donor cells or tissues and analyse. 2) Ligation (joining) of insert and vector DNA molecules together 3) Introduction of insert-vector recombinant construct into suitable host cells 4) Prepare vector DNA in which the target DNA will be inserted 5) Restriction enzyme digestion of target DNA and vector DNA 6) Growth, selection and identification of bacterial host cells containing recombinant DNA
1) Prepare pure insert DNA (target or foreign) samples from donor cells or tissues and analyse. 2) Prepare vector DNA in which the target DNA will be inserted 3) Restriction enzyme digestion of target DNA and vector DNA 4) Ligation (joining) of insert and vector DNA molecules together 5) Introduction of insert-vector recombinant construct into suitable host cells 6) Growth, selection and identification of bacterial host cells containing recombinant DNA
In the UV absorbance Spectrophotometry method, the amount of absorbed light is related to the nucleic acid concentration. This means that by increasing the concentration of nucleic acid, the absorption of UV...
Increases
Decreases
It does not change
None of above
Proteins have a UV max absorption of ~280 nm, due mostly to Phenylalanin residues.
False
True
What do steps 4 and 6 show, respectively?
Combine targeted fragment and plasmid DNA, Genes and proteins are isolated from the cloned bacterium
Cut cell's DNA with same enzyme, Put plasmid into bacterium by transformation
Cut cell's DNA with same enzyme, Add DNA ligase which closes the circle with covalent bonds.
Add DNA ligase which closes the circle with covalent bonds, Cut cell's DNA with same enzyme
Which option is wrong about bacterial plasmid?
Plasmids are small, circular pieces of single-stranded DNA found mainly in bacteria
They are extrachromosomal
They are useful as vectors
They are self-replicating
They carry one or more genes
They range in size from 1 kb to > 500 kb
How works Type II Restriction Enzymes?
They cut DNA by cleaving ionic bonds between adjacent nucleotides in double-stranded DNA
They cut DNA by cleaving phosphodiester bonds between adjacent nucleotides in double-stranded DNA
They cut DNA by cleaving hydrogen bonds between adjacent nucleotides in double-stranded DNA
They cut DNA by cleaving peptide bonds between adjacent nucleotides in double-stranded DNA
Many vectors, such as pUC19, contain the lacZ gene, which codes for:
Dehydrogenase
RNA polymerase
Beta-galactosidase
DNA polymerase
Smaller DNA fragments move faster through the small pores during electrophoresis than larger DNA fragments towards the positive pole.
False
True
This question wasn't directly in the text of the lesson. Please answer based on your guess and the information you have :)what does maximum number of existing transgenic animals belong to?
Pigh
Cow
Mice
Fish
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