Week 1 Revision
Week 1 Revision Quiz: Chromatography & Protein Analysis
Test your knowledge on chromatography and protein analysis with our comprehensive quiz! This quiz covers essential principles, techniques, and calculations involving protein purification and characterization. Challenge yourself to see how much you remember!
- 11 engaging questions
- Multiple choice, checkbox, and text input formats
- Score yourself and improve your understanding
What is the main principle behind chromatography?
Identifying types of protein
Separating components of a mixture
Solubilising components of a mixture
Determining protein concentration
You have a His tagged protein how will you purify it? (Choose the best option)
Affinity Chromatography with Ni bound resin
Size exclusion based upon weight
Charge separation using pI
Select the right statements about pI
When pH=pI Charge of protein=1
When pH=pI Charge of protein=0
When pH>pI Charge of protein is negative
When pH>pI Charge of protein is positive
In size exclusion chromatography:
Larger bands come out first
Smaller bands come out first
Bands come out based on the proteins pI
Bands come out based on the pH of the elution buffer
What is the Beer Lamberts equation. (Note: Re-arranged to find concentration, e is epsilon)
C=A/el
C=Ael
C=el/A
In the Beer Lamberts Law what is the molar extinction coefficient
E (M-1 c-1)
E (M cm-1)
E (M cm)
E (M-1 cm)
You have Purified Protein X. At 1:10 dilution its absorption value at 280nm is 35 and its molar extinction coefficient is 46995 (Use units from previous answer). What is the stock protein concentration?
0.7447mM
0.1644mM
7.447mM
16.44mM
74.47 uM
16.44uM
You have dissolved 10g of NaCl (M.W= 58.44 g/mol) in 100 ml Distilled water (M.W= 18.01g/mol). What is the concentration of the Solution.
5.55 M
0.584 M
0.1801 M
1.711 M
Using the above values, how will you make 1M NaCl solution
58.44g NaCl in 500 ml H20
5.844g NaCl in 1L H20
0.05844 mg in 100 ml H20
58.44 g in 100ml H20
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