Flow Cytometry 101

Create an illustration of a sophisticated laboratory setting with flow cytometry equipment, showcasing colorful fluorescent dyes in action with researchers examining cell samples.

Flow Cytometry Basics

Welcome to the Flow Cytometry 101 quiz! Test your knowledge on fluorophores, lasers, and other essential aspects of flow cytometry. Whether you're a beginner or an experienced professional, this quiz is designed to enhance your understanding of this important technique.

What you'll learn:

  • Key concepts related to flow cytometry
  • Understanding of different fluorophores and their applications
  • Insights into the functionality of flow cytometry instruments
16 Questions4 MinutesCreated by AnalyzingCells101
PC7 and PE-Cy7 are the same fluorophore.
True
False
How many lasers does the Lyric have?
2
5
3
4
Select the tandem dyes.
FITC
PerCP-Cy5.5
PE
APC-Cy7
RY586
BV421
What does Side Scatter (SSC) measure?
What cell type can be found in cluster 1?
What cell type can be found in cluster 2?
What cell type can be found in cluster 3?
What is the purpose of application settings?
How many colors can the Fortessa detect?
15
18
20
13
What staining measures proliferation and has a staining profile as pictured?
You can use CD25 and either Foxp3 or CD127 to mark Tregs
True
False
Which detector would BV421 fall into?
A
B
C
D
E
Which laser and detector would PE fall under? (Emission = 578)
Red B
Blue C
Red C
Blue E
Blue B
What is a tandem dye? (Briefly explain how they work.)
Name:
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