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Quizzes > Health & Medicine

AFI 44-108 quiz: Test your infection control skills

Quick, free infection control competency test with instant results.

Editorial: Review CompletedCreated By: Monica PowellUpdated Aug 24, 2025
Difficulty: Moderate
2-5mins
Learning OutcomesCheat Sheet
Paper art illustration showing mask sanitizer virus with infection control quiz text on golden yellow background

This AFI 44-108 infection control quiz helps you check how you apply TB control, standard and transmission-based precautions, PPE, and exposure response. Fill any gaps with focused practice in our infection control practice test, review basics with a standard precautions quiz, and broaden your knowledge with an infectious diseases quiz.

Easy
What is the primary purpose of Standard Precautions in infection control?
To protect healthcare workers only
To reduce environmental contamination only
To protect both healthcare workers and patients
To protect patients only
Standard Precautions are the foundation of effective infection control, designed to protect both healthcare workers and patients from transmission of infectious agents. They combine elements of universal precautions and body substance isolation. These measures apply to blood, all body fluids, secretions, excretions (except sweat), non-intact skin, and mucous membranes.
Which personal protective equipment is essential when caring for a patient with a known airborne infectious disease?
N95 respirator
Face shield
Surgical mask
Powered air-purifying respirator
An N95 respirator filters out at least 95% of airborne particles and is the minimum requirement for protection against airborne pathogens such as tuberculosis and measles. Surgical masks protect against droplets but do not seal to the face. PAPRs offer equivalent or higher protection but are not the standard first-line device.
According to infection control guidelines, when should hand hygiene be performed?
Every hour
Only before meals
Only after glove removal
Before and after every patient contact
Hand hygiene is the single most effective measure to prevent the spread of infections and should be performed before and after every patient contact, after removing gloves, and after contact with potentially contaminated surfaces. This is a core element of Standard Precautions.
What color is typically designated for biohazard waste bags in healthcare facilities?
Blue
Black
Red
Yellow
Red bags or red containers marked with the universal biohazard symbol are used for disposing of regulated medical waste that may pose a biological hazard. This color coding helps ensure proper handling and disposal.
What is the minimum recommended duration for performing hand hygiene with an alcohol-based hand rub?
60 seconds
10 seconds
30 seconds
20 seconds
An alcohol-based hand rub should be applied and rubbed thoroughly for at least 20 seconds to ensure adequate coverage and microbial kill. Shorter durations may not effectively eliminate pathogens.
Medium
Which of the following cleaning practices is a Category IA recommendation under AFI 44-108 for high-touch environmental surfaces?
Monthly deep cleaning
Daily disinfection of high-touch surfaces
Weekly cleaning
Cleaning only when visibly soiled
Category IA recommendations are strongly supported by experimental or epidemiologic studies. Daily disinfection of high-touch environmental surfaces prevents pathogen transmission. Less frequent or reactive cleaning is not sufficient to control infection risk.
What is the correct disposal method for contaminated sharps in a medical facility?
Puncture-resistant sharps container
Regular trash
Biohazard waste bag
Chemical neutralization in sink
Contaminated sharps must be placed immediately into a designated puncture-resistant sharps container to prevent needlestick injuries. They should never be disposed of in regular trash or chemical sinks.
Which transmission-based precaution is indicated for Clostridioides difficile infection?
Standard precautions only
Droplet precautions
Airborne precautions
Contact precautions
C. difficile is transmitted via spores that survive on surfaces and hands. Contact precautions, including gown and glove use and environmental cleaning with sporicidal agents, are required. Droplet or airborne measures are not indicated unless the disease presentation changes.
According to AFI 44-108 guidelines, what is the minimum required air change rate per hour for airborne infection isolation rooms?
20 air changes per hour
12 air changes per hour
6 air changes per hour
15 air changes per hour
Airborne infection isolation rooms should maintain at least 12 air changes per hour for new or renovated facilities to ensure rapid removal of airborne contaminants. Older facilities may have lower rates but a minimum of six is acceptable.
When sterilizing wrapped instruments in an autoclave, what standard temperature and time must be met?
115°C for 30 minutes
121°C for 5 minutes
121°C for 15 minutes
132°C for 3 minutes
Gravity displacement autoclaves must reach 121°C at 15 psi for at least 15 minutes to achieve sterility of wrapped instruments. Lower temperatures or shorter times do not guarantee kill of all spores.
Hard
How frequently must bloodborne pathogen training be provided to healthcare workers under AFI 44-108?
Quarterly
Bi-annually
Monthly
Annually
Federal regulations mandate annual bloodborne pathogen training to ensure healthcare workers remain current on safe handling and exposure control measures. This frequency reduces the risk of occupational exposure.
If a respirator seal check fails before patient care, what is the appropriate action?
Proceed since a small leak is acceptable
Discard the respirator and select a proper size or type
Tighten the straps further and proceed
Decontaminate and reuse the same respirator
A proper seal is critical for respirator efficacy. If a seal check fails, the respirator must be discarded or replaced with the correct size or model before entering a hazardous environment. Continuing with a poor seal compromises protection.
Which sterilization monitoring method provides immediate visual confirmation during the process?
Chemical indicator
Biological indicator
Radiologic indicator
Mechanical indicator
Chemical indicators change color or form upon exposure to sterilization parameters, offering immediate confirmation that the process conditions were met. Biological indicators require incubation and take longer to confirm sterility.
What concentration of sodium hypochlorite (household bleach) is recommended for disinfecting blood spills?
1:50 bleach solution
1:100 bleach solution
1:10 bleach solution
1:5 bleach solution
A 1:10 dilution of household bleach (approximately 5,000 ppm available chlorine) is effective for disinfecting blood spills by inactivating bloodborne pathogens. Weaker solutions may not fully eliminate spores or viruses.
Transmission-based precautions include all of the following EXCEPT:
Droplet precautions
Contact precautions
Radiation precautions
Airborne precautions
Transmission-based precautions consist of contact, droplet, and airborne precautions to control specific transmission routes. Radiation precautions are unrelated and involve protection from ionizing or non-ionizing radiation.
Expert
Per AFI 44-108, what is the maximum allowable concentration of viable particles in a Class 100 laminar flow hood, measured in particles per cubic foot?
?350 particles/ft³
?500 particles/ft³
?1000 particles/ft³
?100 particles/ft³
Class 100 (ISO Class 5) laminar flow hoods must maintain 100 or fewer particles of size ?0.5 µm per cubic foot of air to ensure a sterile work environment. Higher counts risk contamination.
According to AFI 44-108, how often must water quality be tested in hemodialysis units?
Annually
Quarterly
Monthly
Weekly
Hemodialysis units require stringent water quality monitoring at least monthly to detect endotoxins and microbial contamination, protecting patient safety. More frequent checks may be indicated in certain circumstances.
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Study Outcomes

  1. Understand AFI 44-108 Guidelines -

    Gain a clear grasp of the key provisions and requirements outlined in AFI 44-108 to support effective infection control practices.

  2. Apply Infection Prevention and Control Program Principles -

    Learn how to implement core components of the infection prevention and control program within your healthcare setting for improved safety.

  3. Evaluate Tuberculosis Infection Control Strategies -

    Assess and compare different tuberculosis infection control measures to ensure compliance and minimize transmission risk.

  4. Analyze Real-World Infection Control Scenarios -

    Practice solving case-based challenges drawn from the infection control quiz to strengthen decision-making skills.

  5. Identify Regulatory Compliance and Best Practices -

    Spot critical regulatory requirements and best practices highlighted in healthcare infection control training to maintain high-quality standards.

Cheat Sheet

  1. AFI 44-108 Infection Prevention and Control Program Overview -

    This section lays out the "plan-do-check-act" framework that underpins the AFI 44-108 infection prevention and control program, ensuring continuous improvement in healthcare infection control training. It details leadership responsibilities, resource allocation, and regular program evaluation per CDC guidelines (cdc.gov) and DoD standards. Remember the cycle with the mnemonic "P-D-C-A" (Plan, Do, Check, Act) to streamline policy updates.

  2. Standard and Transmission-Based Precautions -

    Standard Precautions apply to all patient care, while Transmission-Based Precautions (contact, droplet, airborne) tackle specific pathogen risks. A handy tip is "SPIDER": Soap, PPE, Isolation, Distance, Eye protection, Respiratory etiquette. This aligns with WHO and AFI 44-108 guidance on layering precautions to break infection chains.

  3. Tuberculosis Infection Control Strategies -

    AFI 44-108 emphasizes a hierarchy of controls for tuberculosis infection control: administrative (screening), environmental (negative-pressure rooms), and respiratory protection (N95 masks). The "A-E-R-P" mnemonic (Administrative, Environmental, Respiratory, Personal) helps recall each tier. Case studies from the National Institute for Occupational Safety and Health (NIOSH) demonstrate up to 90% risk reduction when all levels are implemented.

  4. Surveillance and Reporting Requirements -

    Effective surveillance under the infection prevention and control program involves active data collection on healthcare-associated infections (HAIs) and timely reporting to relevant public health authorities. Calculate incidence rates using the formula: (Number of new HAI cases ÷ Total patient-days) × 1,000. This metric is central to your infection control quiz prep and aligns with CDC's NHSN reporting standards.

  5. Personal Protective Equipment (PPE) Best Practices -

    Choosing the right PPE (gloves, gowns, eye protection, masks) reduces transmission during high-risk procedures. Follow the "G-MEG" order when donning and doffing (Gown, Mask/Respirator, Eye protection, Gloves) to avoid self-contamination. Regular fit-testing and competency checks are cornerstones of healthcare infection control training per Joint Commission recommendations.

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