3rd test in Molecular Biology

Generate an illustration of a DNA strand intertwined with various laboratory equipment like test tubes, pipettes, and a microscope, set against a vibrant and colorful lab background emphasizing molecular biology themes.

3rd Test in Molecular Biology

Test your knowledge and understanding of Molecular Biology with our engaging quiz! Dive into critical concepts like PCR, genetic mutations, and sequencing techniques.

20 challenging questions
Multiple choice and checkbox formats
Explore the latest in molecular techniques

20 Questions5 MinutesCreated by ExploringGene3

1. During Real-time PCR:

A. Dye terminator is used

B. DNA alterations can be detected on the basis of altered electrophoretic mobility of PCR products

C. Taqman probes can be used

D. Restriction enzymes can be used

2. Mark the correct combinations (technique – detection method):

A. Sanger sequencing – capillary electrophoresis

B. Real-time PCR – fluorescent microscopy

C. PCR – gel electrophoresis

D. NGS – gel electrophoresis

3. Which of the following mutations can lead to colorectal cancer:

A. Deletion of exon 1 in C-ras gene

B. Deletion of 1 nucleotide in intron 5 of MLH1 gene

C. Deletion of 2bp in exon 2 of MSH2 gene

D. Deletion of entire N-myc gene

4. Choose true sentence about electrophoreses:

A. During electrophoresis DNA fragments migrate towards negative electrode

B. Speed of migration relies on size and shape of particles – smaller fragments migrate faster than bigger ones.

C. The observation of electrophoresis is able by addition a dye – bromophenol blue, which migrates together with fragments.

D. Visualization DNA due to added ethidium bromide to the gel

5. MLPA:

A. Can be used in detection of deletion of entire gene

B. Multiplex hybridization and ligation takes place during MLPA analysis

C. Is performed on the basis of genomic DNA

D. Is used in deletion of point mutation

6. Which methods can be used for the identification of large mutations?

A. Taqman Real Time PCR

B. MLPA

C. RFLP

D. NGS

7. Which sentences are true:

A. Cell culture is used in detection of large genomic deletions

B. Capillary electrophoresis is one of the sequencing steps

C. ddNTPs are used during sequencing reaction

D. TaqPolymerase is a human enzyme necessary for translation

8. Which of the following therapies can be used in treatment of inherited breast cancer:

A. Radiotherapy

B. Chemotherapy

C. Mastectomy (surgery)

D. Replacement of BRCA1 gene using artificial mRNA construct

9. Which of the following can cause mutations which contribute to development of cancers?

10. Restriction enzymes used in RFLP-PCR analysis are isolated from:

A. Plants

B. Viruses

C. Fungus

D. Bacteria

11. Which method is preferable for finding new/unknown point mutations:

A. Karyotyping

B. Sequencing

C. NGS

D. RFLP

12. In which databases you can find information about genetic disorders:

A. NCBI

B. OMIM

C. ENSEMBL

D. ORACLE

13. TaqMan probes are used in Real-Time PCR, select correct sentences

A. Labelled: reporter fluorophore –5’ end; quencher fluorophore – 3’ end

B. Cheap and easy to design

C. Their techniques uses dideoxynucleotides

D. The PCR should be proceeded with library preparation

14. What are the advantages of sequencing?

A. Read length up to 100000bp

B. Sensitivity almost 100%

C. Fast and easy

D. Detection of the mutation, from unknown to known

15. Which of the following is characteristic of benign tumor?

A. Gives metastis

B. Slow rate of growth

C. Can be caused by somatic mutations

D. Can be caused by germline mutations

16. Mark techniques used in detection of point mutations:

A. Real-Time PCR

B. Sequencing

C. NGS

D. Karyotyping

17. DNA microsatellites:

a. Are essential for removal of introns during RNA processing

B. Are the target sequences for majority of restriction enzymes

C. Are dispersed in human genome

D. Are located between the genes only

18. Which of the following ingredients can be used for TaqMan PCR analysis?

A. Fluorescent probes

B. SYBR green

C. Ethidium bromide

D. DNA template

19. What are the applications for Real-Time PCR?

A. Protein analysis

B. SNP genotyping

C. Verification of microarray results

D. Identification of viral DNA

20. Select correct sentences for Real-Time PCR?

A. Technique used for determining the nucleotide order of a given DNA fragment

B. Is a technique used to monitor the progress of a PCR reaction in real time

C. Is used for Allelic discrimination assays of SNP genotyping

D. Is used for Targeted Resequencing

3rd test in Molecular Biology

3rd Test in Molecular Biology

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